Kugereranya ingaruka z'ibintu bitera bagiteri na aside gibberellic ku mikurire ya stevia no gukora glycoside ya steviol binyuze mu kugenzura imiterere yayo.

Ubuhinzi ni umutungo w'ingenzi cyane ku masoko y'isi, kandi ibidukikije bihura n'ibibazo byinshi. Ikoreshwa ry'ifumbire mvaruganda ku isi ririmo kwiyongera kandi rigira uruhare runini mu musaruro w'ibihingwa1. Ariko, ibimera bihingwa muri ubu buryo ntibigira umwanya uhagije wo gukura no gukura neza bityo ntibigire ubwiza bwiza bw'ibimera2. Byongeye kandi, ibintu byangiza cyane bishobora kwirundanya mu mubiri w'umuntu no mu butaka3. Kubwibyo, hakenewe gushyiraho ibisubizo birambye kandi birengera ibidukikije kugira ngo bigabanye ikenerwa ry'ifumbire mvaruganda. Udukoko dufasha dushobora kuba isoko y'ingenzi y'ibintu karemano bikora ku binyabuzima4.
Imiterere y’uturemangingo tw’amababi iratandukanye bitewe n’ubwoko bw’ibimera cyangwa ubwoko bwabyo, aho ibimera bikura, n’imiterere y’ibimera. 13 Ubushakashatsi bwinshi bwagaragaje ko Azospirillum, Bacillus, Azotobacter, Pseudomonas, na Enterobacter bifite ubushobozi bwoguteza imbere imikurire y'ibimera. 14 Byongeye kandi, Bacillus na Azospirillum ni zo ngano za PGPB ziganjemo ubushakashatsi bwimbitse mu bijyanye no kunoza imikurire n'umusaruro w'ibimera. 15 Ubushakashatsi bwagaragaje ko gutera Azospirillum brasiliensis na Bradyrhizobium mu binyamisogwe bishobora kongera umusaruro w'ibigori, ingano, soya, n'ibishyimbo by'impyiko. 16, 17 Ubushakashatsi bwagaragaje ko gutera Salicornia hamwe na Bacillus licheniformis n'izindi PGPB bifasha mu mikurire y'ibimera no gufata intungamubiri. 18 Azospirillum brasiliensis Sp7 na Bacillus sphaericus UPMB10 bifasha mu mikurire y'ibitoki biryoshye gukura neza. Mu buryo nk'ubwo, imbuto za fennel ziragoye guhinga bitewe n'imikurire mibi y'ibimera no kumera nabi, cyane cyane iyo habayeho amapfa. 20. Kuvura imbuto hakoreshejwe Pseudomonas fluorescens na Trichoderma harzianum bituma imbuto za fennel zikura vuba mu gihe habayeho amapfa. Kuri stevia, hakozwe ubushakashatsi kugira ngo harebwe ingaruka z'ibihumyo bya mycorrhizal n'imikurire y'ibimera bitera rhizobacteria (PGPR) ku bushobozi bw'ibinyabuzima bwo gukura, kwegeranya metabolites za kabiri, no kugaragaza genes zigira uruhare mu gukora biosynthesis. Nk'uko Rahi et al.22 abivuga, gutera ibimera bifite PGPRs zitandukanye byazamuye imikurire yabyo, imiterere ya photosynthesis, no guteranya stevioside na stevioside A. Ku rundi ruhande, gutera stevia hamwe na rhizobia itera imikurire y'ibimera n'ibihumyo bya arbuscular mycorrhizal byashishikarije uburebure bw'ibimera, stevioside, imyunyungugu, n'ibikubiye mu ibara ry'umuhondo.23 Oviedo-Pereira et al.24 bavuze ko endophytes zitera ishavu Enterobacter hormaechei H2A3 na H5A2 byongera SG, bitera ubucucike bwa trichome mu mababi, kandi bitera kwiyongera kwa metabolites runaka muri trichomes, ariko ntibyateye imbere imikurire y'ibimera;
GA3 ni imwe muri poroteyine zimeze nka gibberellin z’ingenzi kandi zikora mu buryo bw’ibinyabuzima31. Gukoresha GA3 mu kuvura stevia hanze bishobora kongera uburebure bw’igiti no kurabya indabyo32. Ku rundi ruhande, ubushakashatsi bumwe bwagaragaje ko GA3 ari ikintu gitera imbaraga ibimera gukora ibintu bya kabiri nka antioxydants na pigments, kandi ni uburyo bwo kwirinda indwara33.
Isano ya phylogenetic y'uturemangingo ugereranyije n'ubundi bwoko bw'ubwoko. Imibare yo kwinjira kwa GenBank itangwa mu dukubo.
Ibikorwa bya Amylase, cellulase na protease bigaragazwa nk'imirongo isobanutse neza ikikije amakoloni, mu gihe imiterere y'umweru ikikije amakoloni igaragaza imikorere ya lipase. Nkuko bigaragara mu mbonerahamwe ya 2, B. paramycoides SrAM4 ishobora gukora hydrolases zose, mu gihe B. paralicheniformis SrMA3 ishobora gukora enzymes zose uretse cellulase, naho B. licheniformis SrAM2 ikora cellulase gusa.
Ingirabuzimafatizo nyinshi z’ingenzi za mikorobe zagiye zifitanye isano no kwiyongera kwa metabolite ya kabiri mu bimera bivura n’ibihumura neza74. Antioxydants zose za enzymatic n’izitari enzymatic ziyongereye cyane muri S. rebaudiana Shou-2 ugereranije n’uburyo zigenzurwa. Ingaruka nziza za PGPB kuri TPC mu muceri nazo zatangajwe na Chamam et al.75; Byongeye kandi, ibyavuye mu bushakashatsi bwacu bihuye n’ibyavuye muri TPC, TFC, na DPPH muri S. rebaudiana, byatewe n’imikorere ya Piriformospora indica na Azotobacter chroococcum76. TPC na TFC77 byari byinshi cyane mu bimera bya basil byavuwe na mikorobe ugereranyije n’ibimera bitavuwe. Byongeye kandi, kwiyongera kwa antioxydants bishobora kubaho kubera impamvu ebyiri: enzymes za hydrolytic zitera imbaraga uburyo bwo kwirinda ibimera nk’uko mikorobe zitera indwara zifata kugeza igihe ikimera kimenyera kwigarurira bagiteri78. Icya kabiri, PGPB ishobora gukora nk'intandaro yo kwinjizwa kw'ibintu bifatika byakozwe binyuze mu nzira ya shikimate mu bimera byo hejuru na mikorobe 79.
Ibisubizo byagaragaje ko hari isano iri hagati y’umubare w’amababi, imiterere y’uturemangingo n’umusaruro wa SG iyo ubwoko bwinshi bwaterwaga hamwe. Ku rundi ruhande, gutera inshuro ebyiri byari byiza kuruta gutera rimwe mu bijyanye no gukura no gutanga umusaruro w’ibimera.
Enzymes za hydrolytic zabonetse nyuma yo gutera bagiteri ku buryo bwa agar burimo substrate y'icyitegererezo no gutera kuri 28 °C mu gihe cy'iminsi 2-5. Nyuma yo gutera bagiteri ku buryo bwa agar busanzwe, imikorere ya amylase yagaragaye hakoreshejwe umuti wa iodine 100. Umurimo wa selulosi wabonetse hakoreshejwe 0.2% by'amazi ya Congo red reagent hakurikijwe uburyo bwa Kianngam et al. 101. Umurimo wa poroteyine wagaragaye mu bice bisobanutse bikikije koloni zikozwe ku buryo bwa agar y'amata nk'uko byasobanuwe na Cui et al. 102. Ku rundi ruhande, lipase 100 yabonetse nyuma yo gutera kuri Tween agar.