Ubuhinzi nisoko yingenzi kumasoko yisi, kandi sisitemu yibidukikije ihura nibibazo byinshi. Ku isi hose ifumbire mvaruganda iragenda yiyongera kandi igira uruhare runini mu musaruro w’ibihingwa1. Nyamara, ibimera byakuze murubu buryo ntibifite umwanya uhagije wo gukura no gukura neza bityo ntibigire imico myiza yibihingwa2. Byongeye kandi, uburozi bwangiza cyane bushobora kwirundanya mumubiri wumuntu nubutaka3. Niyo mpamvu, hakenewe guteza imbere ibidukikije byangiza ibidukikije kandi birambye kugirango bigabanye ifumbire mvaruganda. Ibinyabuzima bifite akamaro birashobora kuba isoko yingenzi yibinyabuzima bikora 4.
Imiryango ya endofitike mumababi iratandukanye bitewe nubwoko bwibimera cyangwa genotype, ibyiciro bikura, hamwe na morphologie yibimera. 13 Ubushakashatsi bwinshi bwerekanye ko Azospirillum, Bacillus, Azotobacter, Pseudomonas, na Enterobacter bafite ubushobozi bwoguteza imbere imikurire y'ibihingwa. 14 Byongeye kandi, Bacillus na Azospirillum nizo genera yize cyane ya PGPB muburyo bwo kuzamura imikurire yumusaruro. 15 Ubushakashatsi bwerekanye ko gufatanya gutera Azospirillum brasiliensis na Bradyrhizobium mu binyamisogwe bishobora kongera umusaruro w'ibigori, ingano, soya, n'ibishyimbo by'impyiko. 16, 17 Ubushakashatsi bwerekanye ko gutera Salicornia hamwe na Bacillus licheniformis hamwe nizindi PGPBs bifatanyiriza hamwe gutera imbere gukura kwibimera no gufata intungamubiri. 18 Azospirillum brasiliensis Sp7 na Bacillus sphaericus UPMB10 biteza imbere imizi yigitoki kiryoshye. Mu buryo nk'ubwo, imbuto ya fennel iragoye gukura kubera imikurire mibi y’ibimera no kumera gake, cyane cyane mu bihe by’amapfa20. Kuvura imbuto hamwe na fluorescens ya Pseudomonas na Trichoderma harzianum biteza imbere gukura hakiri kare ingemwe za fennel mugihe cy'amapfa21. Kuri stevia, hakozwe ubushakashatsi bwo gusuzuma ingaruka ziterwa na mycorrhizal no gukura kw'ibimera biteza rhizobacteria (PGPR) ku bushobozi bw’ibinyabuzima gukura, kwegeranya metabolite ya kabiri, no kwerekana genes zigira uruhare muri biosynthesis. Nk’uko Rahi et al.22 abivuga, gutera ibiti bifite PGPR zitandukanye byateje imbere imikurire yabyo, indangagaciro ya fotosintezitike, hamwe no kwegeranya stevioside na stevioside A. Ku rundi ruhande, gutera stevia hamwe n’ibiti bikura bikura rhizobia na arbuscular mycorrhizal fungi byateje uburebure bw’ibimera, stevioside, minerval, hamwe na pigment. Enterobacter hormaechei H2A3 na H5A2 yongereye SG ibirimo, itera ubwinshi bwa trichome mumababi, kandi iteza imbere kwegeranya metabolite yihariye muri trichomes, ariko ntabwo byateje imbere ibihingwa;
GA3 nimwe mubyingenzi kandi bikora biologiya ikora gibberelline isa na poroteyine31. Kuvura bidasanzwe bya stevia hamwe na GA3 birashobora kongera kuramba no kurabyo32. Ku rundi ruhande, ubushakashatsi bumwe na bumwe bwerekanye ko GA3 ari inducer itera ibimera gukora metabolite ya kabiri nka antioxydants na pigment, kandi nuburyo bwo kwirwanaho33.
Umubano wa phylogeneque wigunga ugereranije nubundi bwoko bwingutu. Imibare yo kwinjira ya GenBank itangwa mumutwe.
Ibikorwa bya Amylase, selile na protease byerekanwe nkibipande bisobanutse bikikije koloni, mugihe imvura yera ikikije koloni yerekana ibikorwa bya lipase. Nkuko bigaragara mu mbonerahamwe ya 2, B. paramycoides SrAM4 irashobora kubyara hydrolase zose, mugihe B. paralicheniformis SrMA3 ishobora gutanga imisemburo yose usibye selile, na B. licheniformis SrAM2 itanga selile gusa.
Ubwoko butandukanye bwa mikorobe bwagiye bujyana no kongera metabolite ya kabiri ya metabolite mu bimera bivura imiti. Antioxydants zose zidasanzwe kandi zidafite imisemburo ziyongereye cyane muri S. rebaudiana Shou-2 ugereranije no kugenzura. Ingaruka nziza ya PGPB kuri TPC mumuceri nayo yavuzwe na Chamam et al.75; Byongeye kandi, ibisubizo byacu bihuye nibisubizo bya TPC, TFC, na DPPH muri S. rebaudiana, byatewe nigikorwa cyahujwe na Piriformospora indica na Azotobacter chroococcum76. TPC na TFC77 byari hejuru cyane mubihingwa bya basile bivurwa na mikorobe ugereranije nibihingwa bitavuwe. Byongeye kandi, kwiyongera kwa antioxydants bishobora kubaho kubwimpamvu ebyiri: imisemburo ya hydrolytique itera uburyo bwo kwirinda ibimera biterwa na mikorobe itera indwara kugeza igihe igihingwa kimenyereye ubukoroni78. Icya kabiri, PGPB irashobora gukora nk'intangiriro yo kwinjiza ibinyabuzima byangiza umubiri binyuze mu nzira ya shikimate mu bimera byo hejuru na mikorobe 79.
Ibisubizo byerekanaga ko hari isano iri hagati yumubare wibabi, imvugo ya gene numusaruro wa SG mugihe amoko menshi yafatanyirijwe hamwe. Ku rundi ruhande, gutera inshuro ebyiri byasumbaga inshinge imwe mu bijyanye no gukura kw'ibihingwa n'umusaruro.
Imisemburo ya Hydrolytique yamenyekanye nyuma yo gukingira bagiteri ku gipimo cya agar kirimo insimburangingo na incubation kuri 28 ° C mu minsi 2-5. Nyuma yo gushira bagiteri kuri krahisi ya agar, ibikorwa bya amylase byagaragaye hakoreshejwe igisubizo cya iyode 100. Igikorwa cya Cellulase cyagenwe hakoreshejwe 0.2% y'amazi yo muri congo itukura reagent ukurikije uburyo bwa Kianngam nabandi. 101. Igikorwa cya poroteyine cyagaragaye binyuze muri zone zisobanutse hafi ya koloni zometse ku mata agar mata agar nkuko byasobanuwe na Cui n'abandi. 102. Kurundi ruhande, lipase 100 yagaragaye nyuma yo gukingirwa hagati ya Tween agar medium.
Igihe cyo kohereza: Mutarama-06-2025