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Exogenous gibberellic aside na benzylamine ihindura imikurire na chimie ya Schefflera dwarfis: isesengura ryintambwe

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Ibiti byamababi meza kandi bifite isura nziza bihabwa agaciro gakomeye. Inzira imwe yo kubigeraho ni ugukoreshaibimera bikurank'ibikoresho byo gucunga ibihingwa. Ubushakashatsi bwakorewe kuri Schefflera dwarf (igihingwa cyamababi yumurimbo) cyavuwe nibiti bya foliar yaacide gibberellicimisemburo ya benzyladenine muri pariki ifite uburyo bwo kuhira imyaka. Umusemburo watewe ku mababi ya dwarf schefflera ku gipimo cya 0, 100 na 200 mg / l mu byiciro bitatu buri minsi 15. Ubushakashatsi bwakozwe ku buryo bufatika muburyo buteganijwe rwose hamwe na bine. Guhuza aside ya gibberellic na benzyladenine ku gipimo cya 200 mg / l byagize ingaruka zikomeye ku mubare w’amababi, ahantu h’ibabi no ku burebure bw’ibiti. Ubu buvuzi kandi bwavuyemo ibintu byinshi biri mu mafoto ya fotosintetike. Byongeye kandi, ibipimo byinshi bya karubone nziza kandi bigabanya isukari byagaragaye hamwe na benzyladenine kuri 100 na 200 mg / L na aside gibberellic + benzyladenine kuri 200 mg / L. Isesengura ryisubiramo ryerekana ko ingano yumuzi aribwo bwa mbere bwahindutse bwinjira muri moderi, busobanura 44% yo gutandukana. Ihinduka ryakurikiyeho ryari imizi mishya, hamwe na bivariate moderi isobanura 63% yo gutandukana mumibare yamababi. Ingaruka nziza cyane kumubare wibabi yatewe nuburemere bushya bwimizi (0.43), bwari bufitanye isano numubare wibabi (0.47). Ibisubizo byagaragaje ko aside ya gibberellic na benzyladenine ku gipimo cya 200 mg / l byateje imbere cyane imikurire y’imiterere, chlorophyll na carotenoid synthesis ya Liriodendron tulipifera, kandi bigabanya ibirimo isukari hamwe na karubone nziza.
Schefflera arborescens (Hayata) Merr ni igihingwa cyimeza cyatsi kibisi cyumuryango wa Araliaceae, kavukire mubushinwa na Tayiwani1. Iki gihingwa gikunze guhingwa nkurugo, ariko igihingwa kimwe gusa gishobora gukura mubihe nkibi. Amababi afite kuva kumpapuro 5 kugeza kuri 16, buri cm 10-20 z'uburebure. Dwarf Schefflera igurishwa ku bwinshi buri mwaka, ariko uburyo bwo guhinga bwa kijyambere ntibukoreshwa gake. Kubwibyo, gukoresha ibiyobora ibihingwa nkibikoresho bifatika byo gucunga neza iterambere no kongera umusaruro urambye wibikomoka ku buhinzi bwimbuto bisaba kwitabwaho cyane. Muri iki gihe, imikoreshereze y’imikurire y’ibihingwa yiyongereye cyane 3,4,5. Acide ya Gibberellic nigenzura ryikura ryibihingwa bishobora kongera umusaruro wibihingwa6. Imwe mu ngaruka zizwi ni ugutera imikurire y'ibimera, harimo kuramba no kurandura imizi hamwe no kongera amababi7. Ingaruka zikomeye za gibberelline niyongera muburebure bwuruti bitewe no kwagura interode. Gutera amababi ya gibberelline ku bimera bya dwarf bidashobora kubyara gibberelline bituma kwiyongera kuramba hamwe nuburebure bwibiti8. Gutera amababi yindabyo namababi hamwe na aside ya gibberellic yibitseho mg / l 500 bishobora kongera uburebure bwibiti, umubare, ubugari nuburebure bwamababi9. Gibberelline yavuzwe ko itera imikurire y'ibiti bitandukanye bigari10. Kurambura uruti byagaragaye muri pinusi ya Scots (Pinussylvestris) na spuce yera (Piceaglauca) igihe amababi yaterwaga acide gibberellic11.
Ubushakashatsi bumwe bwasuzumye ingaruka ziterwa na sitokinine eshatu zikura zikura kumashami yinyuma muri Lily officinalis. kugoreka Ubushakashatsi bwakozwe mugihe cyizuba nimpeshyi kugirango bige ingaruka zigihe. Ibisubizo byerekanaga ko kinetin, benzyladenine na 2-prenyladenine bitagize ingaruka ku mashami yinyongera. Nyamara, 500 ppm benzyladenine yatumye hashyirwaho amashami yingoboka 12.2 na 8.2 mubushakashatsi bwimpeshyi nimpeshyi, ugereranije namashami 4.9 na 3.9 mubiti bigenzura. Ubushakashatsi bwerekanye ko kuvura impeshyi bigira akamaro kuruta igihe cy'itumba12. Mu bundi bushakashatsi, Amahoro Lily var. Ibihingwa bya Tassone byavuwe hamwe na 0, 250 na 500 ppm benzyladenine mumasafuriya ya cm 10. Ibisubizo byerekanye ko gutunganya ubutaka byongereye cyane umubare wamababi yinyongera ugereranije no kugenzura nibihingwa bivangwa na benzyladenine. Amababi mashya yinyongera yagaragaye nyuma yibyumweru bine nyuma yo kuvurwa, kandi umusaruro mwinshi wamababi wagaragaye nyuma yibyumweru umunani nyuma yo kuvurwa. Mugihe cibyumweru 20 nyuma yubuvuzi, ibihingwa bivangwa nubutaka byagize uburebure buke ugereranije nibihingwa byabanje kuvurwa13. Byagaragaye ko benzyladenine yibumbiye kuri 20 mg / L ishobora kongera cyane uburebure bwibiti n’umubare w’ibabi muri Croton 14. Muri lili ya calla, benzyladenine kuri 500 ppm byatumye umubare w’amashami wiyongera, mu gihe umubare y'amashami yari make mumatsinda yo kugenzura15. Icyari kigamijwe muri ubu bushakashatsi kwari ugukora iperereza ku gutera amababi ya aside ya gibberellic na benzyladenine kugira ngo imikurire ya Schefflera dwarfa, igihingwa cy’imitako. Ibi bigenga imikurire yibihingwa birashobora gufasha abahinzi mubucuruzi gutegura umusaruro ukwiye umwaka wose. Nta bushakashatsi bwakozwe kugirango iterambere rya Liriodendron tulipifera.
Ubu bushakashatsi bwakorewe muri parike yubushakashatsi bwibimera muri kaminuza ya kisilamu Azad i Jiloft, muri Irani. Gutera imizi imwe ya dwarf schefflera ya cm 25 ± 5 z'uburebure byateguwe (bikwirakwizwa amezi atandatu mbere yubushakashatsi) hanyuma bibibwa mu nkono. Inkono ni plastiki, umukara, ifite diameter ya cm 20 n'uburebure bwa cm 30.
Umuco wo muri ubu bushakashatsi wari uruvange rwa peat, humus, umucanga wogejwe hamwe numuceri wumuceri ku kigereranyo cya 1: 1: 1: 1 (kubunini) 16. Shira igiti cy'amabuye hepfo yinkono kugirango amazi. Ikigereranyo cy'ubushyuhe bwo ku manywa na nijoro muri parike mu mpeshyi no mu cyi byari 32 ± 2 ° C na 28 ± 2 ° C. Ubushuhe bugereranije bugera kuri> 70%. Koresha sisitemu yo kubeshya. Ugereranije, ibimera bivomera inshuro 12 kumunsi. Mu gihe cyizuba nimpeshyi, igihe cyo kuvomera ni iminota 8, intera yo kuvomera ni isaha 1. Ibimera na byo byakuze inshuro enye, ibyumweru 2, 4, 6 na 8 nyuma yo kubiba, hamwe n'umuti wa micronutrient (Ghoncheh Co, Irani) ku gipimo cya saa tatu za mugitondo hanyuma ukavomererwa na ml 100 yumuti buri gihe. Igisubizo cyintungamubiri kirimo N 8 ppm, P 4 ppm, K 5 ppm hamwe nibintu bya Fe, Pb, Zn, Mn, Mo na B.
Ibice bitatu bya acide ya gibberellic hamwe nubugenzuzi bwikura ryibihingwa benzyladenine (byaguzwe muri Sigma) byateguwe kuri 0, 100 na 200 mg / L hanyuma bigaterwa kumuti wibiti mubyiciro bitatu mugihe cyiminsi 1517. Hagati ya 20 (0.1%) (yaguzwe na Sigma) yakoreshejwe mugisubizo kugirango yongere kuramba no kugabanuka. Mu gitondo cya kare, shyira imisemburo ku mababi n'ibibabi bya Liriodendron tulipifera ukoresheje sprayer. Ibimera byatewe namazi yatoboye.
Uburebure bwibiti, diameter yibiti, agace k'ibabi, ibirungo bya chlorophyll, umubare wa internode, uburebure bwamashami yisumbuye, umubare wamashami yisumbuye, ubwinshi bwumuzi, uburebure bwumuzi, ubwinshi bwibabi, imizi, igiti nibintu byumye, ibirimo pigment ya fotosintetike (chlorophyll) a, chlorophyll b) Chlorophyll yose, karotenoide, pigment zose), kugabanya isukari hamwe na karubone nziza ya elegitoronike byapimwe mubuvuzi butandukanye.
Ibirimo bya chlorophyll byamababi akiri muto byapimwe nyuma yiminsi 180 nyuma yo gutera ukoresheje metero ya chlorophyll (Spad CL-01) guhera 9h30 kugeza 10h00 (kubera gushya kwamababi). Byongeye kandi, ahantu h’ibabi hapimwe nyuma yiminsi 180 nyuma yo gutera. Gupima amababi atatu uhereye hejuru, hagati no hepfo yuruti kuri buri nkono. Aya mababi noneho akoreshwa nkicyitegererezo kurupapuro rwa A4 hanyuma ibisubizo bivamo bigacibwa. Uburemere n'ubuso bw'urupapuro rumwe rw'impapuro A4 nabyo byapimwe. Noneho ubuso bwibibabi byafashwe bibarwa ukoresheje ibipimo. Byongeye kandi, ingano yumuzi yagenwe hakoreshejwe silinderi yarangije. Uburemere bwamababi yumye, uburemere bwumye, uburemere bwumuzi, nuburemere bwumye bwa buri cyitegererezo byapimwe no kumisha ifuru kuri 72 ° C mumasaha 48.
Ibiri muri chlorophyll na karotenoide byapimwe nuburyo bwa Lichtenthaler18. Kugirango ukore ibi, 0.1 g yamababi mashya yashizwe mumabuye ya farashi arimo ml 15 ya 80% ya acetone, hanyuma nyuma yo kuyungurura, ubucucike bwa optique bwapimwe hifashishijwe spekitifotometero kumuraba wa 663.2, 646.8 na 470 nm. Hindura igikoresho ukoresheje 80% acetone. Kubara ubunini bwibintu bya fotosintetike ukoresheje ikigereranyo gikurikira:
Muri byo, Chl a, Chl b, Chl T na Imodoka byerekana chlorophyll a, chlorophyll b, chlorophyll yose hamwe na karotenoide. Ibisubizo bitangwa mu gihingwa cya mg / ml.
Kugabanya isukari byapimwe hakoreshejwe uburyo bwa Somogy19. Kugirango ukore ibi, 0,02 g yibiti byibimera bishyirwa mumabuye ya farashi hamwe na ml 10 y'amazi yatoboye hanyuma agasukwa mubirahuri bito. Shyushya ikirahuri kubira hanyuma ushungure ibirimo ukoresheje urupapuro rwa Whatman No 1 rwungurura kugirango ubone ibimera. Hindura ml 2 ya buri gikururwa mumiyoboro yipimisha hanyuma wongeremo ml 2 yumuti wa sulfate wumuringa. Gupfundikira umuyoboro wipimisha ubwoya bwipamba hanyuma ushushe mubwogero bwamazi kuri 100 ° C muminota 20. Kuri iki cyiciro, Cu2 + ihindurwamo Cu2O kugabanya aldehyde monosaccharide kandi ibara rya salmon (terracotta) rigaragara hepfo yigitereko. Umuyoboro wikizamini umaze gukonja, ongeramo ml 2 ya acide fosifomolibdic hanyuma ibara ryubururu rigaragare. Shyira umuyoboro cyane kugeza ibara ryagabanijwe neza muri tube. Soma ibyinjira mubisubizo kuri 600 nm ukoresheje spekitifotometero.
Kubara intumbero yo kugabanya isukari ukoresheje umurongo usanzwe. Ubushuhe bwa karubone ya elegitoronike yashizweho nuburyo bwa Fales20. Kugirango ukore ibi, 0.1 g yimimero yavanze na ml 2,5 ya 80% Ethanol kuri 90 ° C kuminota 60 (ibyiciro bibiri bya 30 min buri) kugirango ikuremo karubone nziza. Ibikuramo noneho birayungurura hanyuma inzoga zishiramo umwuka. Imvura ivamo irashonga muri ml 2,5 y'amazi yatoboye. Suka ml 200 ya buri cyitegererezo mumiyoboro yipimisha hanyuma wongeremo ml 5 yerekana anthrone. Uruvange rwashyizwe mu bwogero bw’amazi kuri 90 ° C mu minota 17, hanyuma nyuma yo gukonja, iyinjira ryayo ryagenwe kuri 625 nm.
Ubushakashatsi bwari igeragezwa rishingiye ku gishushanyo mbonera cyuzuye hamwe na bine. Gahunda ya PROC UNIVARIATE ikoreshwa mugusuzuma ubusanzwe ikwirakwizwa ryamakuru mbere yo gusesengura ibitandukanye. Isesengura mibare ryatangiranye nisesengura ryibarurishamibare risobanura kugirango wumve ubwiza bwamakuru yibanze yakusanyijwe. Ibiharuro byashizweho kugirango byoroshe kandi bigabanye amakuru manini kugirango byoroshye kubisobanura. Nyuma, hasesenguwe byinshi bigoye. Ikizamini cya Duncan cyakozwe hakoreshejwe software ya SPSS (verisiyo ya 24; IBM Corporation, Armonk, NY, USA) kugirango ibare kare kare hamwe namakosa yubushakashatsi kugirango hamenyekane itandukaniro riri hagati yamakuru. Ikizamini cya Duncan cyinshi (DMRT) cyakoreshejwe kugirango hamenyekane itandukaniro riri hagati yuburyo bugaragara (0.05 ≤ p). Coefficient ya Pearson (r) yabazwe ikoresheje software ya SPSS (verisiyo ya 26; IBM Corp., Armonk, NY, USA) kugirango isuzume isano riri hagati yimpande zitandukanye. Mubyongeyeho, isesengura ryumurongo wo kugaruka ryakozwe hifashishijwe software ya SPSS (v.26) kugirango hamenyekane indangagaciro zumwaka wambere uhindagurika ukurikije indangagaciro zumwaka wa kabiri. Ku rundi ruhande, isesengura ryo gusubira inyuma hamwe na p <0.01 ryakozwe kugirango hamenyekane imico igira ingaruka zikomeye kumababi ya dwarf schefflera. Isesengura ryinzira ryakozwe kugirango hamenyekane ingaruka zitaziguye kandi zitaziguye za buri kiranga murugero (hashingiwe kubiranga bisobanura neza itandukaniro). Ibiharuro byose byavuzwe haruguru (ubusanzwe bwo gukwirakwiza amakuru, coefficente yoroheje ihuza, gusubira inyuma no gusesengura inzira) byakozwe hakoreshejwe software ya SPSS V.26.
Ingero z’ibihingwa byatoranijwe byahujwe n’amabwiriza abigenga, y’igihugu ndetse n’amahanga ndetse n’amategeko yo muri Irani.
Imbonerahamwe 1 irerekana imibare isobanura ibisobanuro, gutandukana bisanzwe, byibuze, ntarengwa, intera, na coefficente ya fenotipiki yo gutandukana (CV) kubintu bitandukanye. Muri iyi mibare, CV yemerera kugereranya ibiranga kuko idafite urugero. Kugabanya isukari (40.39%), uburemere bwumuzi (37.32%), uburemere bushya bwumuzi (37,30%), isukari ku kigereranyo cyisukari (30.20%) nubunini bwumuzi (30%) nibyo hejuru. n'ibirimo bya chlorophyll (9,88%). ) hamwe nibibabi bifite indangagaciro ndende (11,77%) kandi bifite agaciro gake CV. Imbonerahamwe 1 irerekana ko uburemere bwuzuye butose bufite intera ndende. Ariko, iyi mico ntabwo ifite CV yo hejuru. Kubwibyo, ibipimo bitagira urugero nka CV bigomba gukoreshwa kugereranya ibiranga impinduka. CV ndende yerekana itandukaniro rinini hagati yo kuvura iyi mico. Ibyavuye muri ubu bushakashatsi byagaragaje itandukaniro rinini hagati yo kuvura isukari nke mu buremere bwumye, uburemere bwumuzi mushya, karubone-hydrata-isukari, hamwe nubunini bwumuzi.
Ibisubizo bya ANOVA byagaragaje ko ugereranije no kugenzura, gutera amababi hamwe na aside ya gibberellic na benzyladenine byagize ingaruka zikomeye ku burebure bw’ibimera, umubare w’amababi, ahantu h’ibabi, ubwinshi bwimizi, uburebure bwumuzi, indangagaciro ya chlorophyll, uburemere bushya nuburemere bwumye.
Kugereranya indangagaciro zisobanutse byerekanaga ko kugenzura imikurire yikimera byagize ingaruka zikomeye kuburebure bwibiti n'umubare w'amababi. Uburyo bwiza bwo kuvura ni aside ya gibberellic yibanze kuri 200 mg / l na aside gibberellic + benzyladenine kuri 200 mg / l. Ugereranije no kugenzura, uburebure bwibihingwa numubare wamababi byiyongereyeho inshuro 32,92 ninshuro 62.76 (Imbonerahamwe 2).
Agace k'amababi kiyongereye cyane muburyo bwose ugereranije no kugenzura, hamwe n'ubwiyongere ntarengwa bwagaragaye kuri 200 mg / l kuri aside ya gibberellic, igera kuri cm 89.19. Ibisubizo byerekanaga ko agace k'ibabi kiyongereye cyane hamwe no kongera imikurire yo kugenzura imikurire (Imbonerahamwe 2).
Ubuvuzi bwose bwongereye cyane ingano yumuzi nuburebure ugereranije no kugenzura. Gukomatanya aside ya gibberellic + benzyladenine yagize ingaruka zikomeye, yongera ubunini nuburebure bwumuzi igice kimwe ugereranije no kugenzura (Imbonerahamwe 2).
Indangagaciro zisumba izindi za diameter nuburebure bwa internode byagaragaye mugucunga na aside ya gibberellic + benzyladenine 200 mg / l ivura.
Indangantego ya chlorophyll yiyongereye muburyo bwose ugereranije no kugenzura. Agaciro kari hejuru yiyi mico kagaragaye mugihe kavuwe na aside ya gibberellic + benzyladenine 200 mg / l, yari hejuru ya 30.21% kuruta kugenzura (Imbonerahamwe 2).
Ibisubizo byerekanye ko ubuvuzi bwatumye habaho itandukaniro rigaragara mubintu bya pigment, kugabanuka kwisukari hamwe na karubone nziza.
Umuti hamwe na aside ya gibberellic + benzyladenine yatumye habaho ibintu byinshi byamafoto ya fotosintetike. Iki kimenyetso cyari hejuru cyane muburyo bwose kuruta kugenzura.
Ibisubizo byerekanaga ko uburyo bwose bwo kuvura bushobora kongera chlorophyll yibirimo bya Schefflera. Nyamara, agaciro gakomeye k’iyi mico kagaragaye mu kuvura hakoreshejwe aside ya gibberellic + benzyladenine, yari hejuru ya 36,95% ugereranije n’ubugenzuzi (Imbonerahamwe 3).
Ibisubizo bya chlorophyll b byari bisa rwose nibisubizo bya chlorophyll a, itandukaniro ryonyine kwari ukwiyongera mubirimo chlorophyll b, byari hejuru ya 67.15% ugereranije no kugenzura (Imbonerahamwe 3).
Ubuvuzi bwatumye hiyongeraho chlorophyll yose ugereranije no kugenzura. Umuti hamwe na aside ya gibberellic 200 mg / l + benzyladenine 100 mg / l yatumye habaho agaciro gakomeye kiyi mico, kari hejuru ya 50% kuruta kugenzura (Imbonerahamwe 3). Ukurikije ibisubizo, kugenzura no kuvura hamwe na benzyladenine ku gipimo cya 100 mg / l byatumye igipimo kinini cy’iyi mico. Liriodendron tulipifera ifite agaciro gakomeye ka karotenoide (Imbonerahamwe 3).
Ibisubizo byerekanye ko iyo bivuwe na aside ya gibberellic yibanda kuri mg / L 200, ibirimo chlorophyll byiyongereye cyane kuri chlorophyll b (Ishusho 1).
Ingaruka ya aside ya gibberellic na benzyladenine kuri a / b Ch. Ingano ya dwarf schefflera. (GA3: aside gibberellic na BA: benzyladenine). Inyuguti zimwe muri buri shusho zerekana ko itandukaniro ridafite akamaro (P <0.01).
Ingaruka ya buri muti ku buremere bushya kandi bwumye bwibiti bya dwarf schefflera byari hejuru cyane ugereranije nubugenzuzi. Acide ya Gibberellic + benzyladenine kuri 200 mg / L niyo miti ivura neza, yongera ibiro bishya kuri 138.45% ugereranije no kugenzura. Ugereranije no kugenzura, uburyo bwose bwo kuvura usibye 100 mg / L benzyladenine bwongereye cyane ibiro byumye byumye, na 200 mg / L aside gibberellic aside + benzyladenine byatumye habaho agaciro gakomeye kuriyi mico (Imbonerahamwe 4).
Ibyinshi mubihinduka byari bitandukanye cyane nubugenzuzi muriki cyerekezo, hamwe nagaciro gakomeye ka 100 na 200 mg / l benzyladenine na 200 mg / l acide gibberellic + benzyladenine (Ishusho 2).
Ingaruka ya aside ya gibberellic na benzyladenine ku kigereranyo cya karubone nziza kandi igabanya isukari muri dwarf schefflera. (GA3: aside gibberellic na BA: benzyladenine). Inyuguti zimwe muri buri shusho zerekana ko nta tandukaniro rinini (P <0.01).
Intambwe yo gusubira inyuma yakozwe kugirango hamenyekane ibiranga nyirizina no gusobanukirwa neza isano iri hagati yibihinduka byigenga numubare wibabi muri Liriodendron tulipifera. Ingano yimizi niyo yambere ihinduka yinjiye murugero, isobanura 44% yo gutandukana. Ibikurikira byahinduwe byari uburemere bwumuzi, kandi izi mpinduka zombi zasobanuye 63% byimpinduka mumibare yamababi (Imbonerahamwe 5).
Isesengura ryinzira ryakozwe kugirango dusobanure neza gusubira inyuma (Imbonerahamwe 6 nishusho 3). Ingaruka nziza nziza kumubare wibabi yahujwe nubwinshi bwimizi (0.43), yari ifitanye isano neza numubare wibabi (0.47). Ibi byerekana ko iyi mico igira ingaruka ku buryo butaziguye ku musaruro, mu gihe ingaruka zayo zitaziguye binyuze mu zindi mico ari ntagaciro, kandi ko iyo mico ishobora gukoreshwa nk'igipimo cyo gutoranya muri gahunda yo korora gahunda ya dwarf schefflera. Ingaruka itaziguye yubunini bwumuzi yari mbi (−0.67). Ingaruka yiyi mico kumubare wamababi iragaragara, ingaruka itaziguye ntabwo ari nto. Ibi byerekana ko uko ingano yumuzi nini, umubare muto wamababi.
Igicapo 4 kirerekana impinduka mumurongo ugaruka kumubare wumuzi no kugabanya isukari. Ukurikije coefficient de regression, buri gice gihinduka muburebure bwumuzi hamwe na karubone ya elegitoronike isobanura bivuze ko ingano yumuzi no kugabanya isukari ihinduka 0.6019 na 0.311.
Coefficient ya Pearson yerekana imiterere yikura irerekanwa mumashusho 5. Ibisubizo byerekanaga ko umubare wamababi nuburebure bwibimera (0.379 *) byari bifite isano ryiza kandi bifite akamaro.
Shyushya ikarita yumubano hagati yimihindagurikire yikigereranyo cyo gukura. # Y Axis: 1-Ironderero Ch., 2-Internode, 3-LAI, 4-N yamababi, 5-Uburebure bwamaguru, 6-diameter. # Kuruhande rwa X axis: A - indangagaciro H., B - intera iri hagati yumutwe, C - LAY, D - N. yikibabi, E - uburebure bwikirenge cy ipantaro, F - diameter yikibaho.
Coefficient ya Pearson kubiranga uburemere bwibintu byerekanwe mubishusho 6. Ibisubizo byerekana isano iri hagati yuburemere bwibabi ryibabi nuburemere bwumye bwo hejuru (0.834 **), uburemere bwumye (0.913 **) nuburemere bwumye bwumuzi (0.562 * ). . Ubwinshi bwumye bufite isano iri hejuru kandi yingenzi ifitanye isano no kurasa byumye (0.790 **) hamwe numuti wumye (0.741 **).
Shyushya ikarita yumubano hagati yuburemere bushya bujyanye na coefficient variable. # Y axis: 1 - uburemere bwamababi mashya, 2 - uburemere bwimbuto nshya, 3 - uburemere bwimizi mishya, 4 - uburemere bwamababi mashya. # X-axis yerekana: A - uburemere bwibabi bushya, B - uburemere bushya, CW - uburemere bwumuzi mushya, D - uburemere bushya.
Coefficient ya Pearson kubintu byumye bifitanye isano nuburemere bwerekanwe mubishusho 7. Ibisubizo byerekana ko uburemere bwamababi yumye, uburemere bwumye (0.848 **) nuburemere bwumye (0.947 **), uburemere bwumye (0,854 **) hamwe na misa yumye yose (0,781 **) ifite indangagaciro zisumba izindi. ihuriro ryiza kandi rifitanye isano.
Shyushya ikarita yumubano hagati yuburemere bwumubyimba uhuza coefficient variable. # Y axis igereranya: uburemere bwibabi-amababi 1, uburemere bwumye 2, uburemere bwumuzi 3, uburemere bwumye 4. # X Axis: Uburemere bw-amababi yumye, B-bud yumye yumye, CW umuzi wumye, D-uburemere bwumye.
Coefficient ya Pearson yumutungo wa pigment irerekanwa mumashusho 8. Ibisubizo byerekana ko chlorophyll a na chlorophyll b (0.716 **), chlorophyll yose (0,968 **) hamwe na pigment zose (0.954 **); chlorophyll b na chlorophyll yose (0.868 **) hamwe na pigment zose (0,851 **); chlorophyll yose ifite ihuriro ryiza cyane kandi rifite akamaro hamwe na pigment zose (0,984 **).
Shyushya ikarita yumubano hagati ya chlorophyll ihuriro coefficient variable. # Y ishoka: 1- Umuyoboro a, 2- Umuyoboro. b, 3 - igipimo cya a / b, imiyoboro 4. Igiteranyo, 5-karotenoide, pigment 6-itanga umusaruro. # X-Axes: A-Ch. aB-Ch. b, igipimo cya C- a / b, D-Ch. Ibirimo byose, E-karotenoide, F-umusaruro wibintu.
Dwarf Schefflera ni urugo ruzwi cyane ku isi, kandi imikurire yarwo n'iterambere byarimo kwitabwaho cyane. Imikoreshereze yubuyobozi bukura bwibimera byatumye habaho itandukaniro rigaragara, hamwe nubuvuzi bwose bwongera uburebure bwibimera ugereranije no kugenzura. Nubwo uburebure bwibimera bugenzurwa genetiki, ubushakashatsi bwerekana ko gushyira mubikorwa kugenzura imikurire yikimera bishobora kongera cyangwa kugabanya uburebure bwibimera. Uburebure bw'ibihingwa n'umubare w'amababi yavuwe hamwe na aside ya gibberellic + benzyladenine 200 mg / L niyo yari hejuru cyane, igera kuri cm 109 na 38.25. Bihuye nubushakashatsi bwabanje (SalehiSardoei et al.52) na Spathiphyllum23, kwiyongera nkuburebure bwibimera bitewe no kuvura aside ya gibberellic byagaragaye muri marigolds yabumbwe, albus alba21, daylilies22, umunsi wumunsi, agarwood na lili yamahoro.
Acide ya Gibberellic (GA) igira uruhare runini mubikorwa bitandukanye byimiterere yibimera. Zitera amacakubiri, kurambura ingirabuzimafatizo, kurambura uruti no kwiyongera 24. GA itera amacakubiri no kurambura mu kurasa apices na meristems25. Guhindura amababi harimo no kugabanya umubyimba wuruti, ingano yamababi mato, hamwe nicyatsi kibisi 26. Ubushakashatsi bwifashishije ibintu bibuza cyangwa bitera imbaraga byerekanye ko calcium ion ziva mumbere zikora nkintumwa za kabiri muri gibberellin yerekana inzira mumasaka corolla27. HA yongerera uburebure bwibimera itera synthesis yimisemburo itera urukuta rwingirabuzimafatizo, nka XET cyangwa XTH, expansins na PME28. Ibi bitera ingirabuzimafatizo kwiyongera uko urukuta rw'akagari ruruhuka kandi amazi yinjira muri selire29. Gukoresha GA7, GA3 na GA4 birashobora kongera kuramba 30,31. Acide ya Gibberellic itera kurambura uruti mu bimera bya dwarf, no mu bimera bya rosette birinda gukura kwamababi no kurambura internode32. Nyamara, mbere yicyiciro cyimyororokere, uburebure bwuruti bwiyongera kugera kuri 4-5 uburebure bwumwimerere33. Inzira ya GA biosynthesis mu bimera yavuzwe mu gishushanyo cya 9.
GA biosynthesis mu bimera n'urwego rwa endogenous bioactive GA, igishushanyo mbonera cy'ibimera (iburyo) na GA biosynthesis (ibumoso). Imyambi ni ibara ryanditse kugirango rihuze nuburyo bwa HA bwerekanwe kumuhanda wa biosynetique; imyambi itukura yerekana igabanuka rya GC bitewe nubusanzwe mu ngingo z’ibimera, naho imyambi yumukara yerekana urwego rwa GC rwiyongereye. Mu bimera byinshi, nk'umuceri na watermelon, ibirimo GA biri hejuru munsi cyangwa igice cyibabi30. Byongeye kandi, raporo zimwe zerekana ko ibinyabuzima bya bioactive GA bigabanuka uko amababi arambuye kuva shingiro34. Urwego nyarwo rwa gibberelline muribi bihe ntiruzwi.
Igenzura ryikura ryibimera naryo rigira uruhare runini mumibare nubuso bwamababi. Ibisubizo byerekanaga ko kongera ubwiyongere bwikura ryikura ryibimera byatumye ubwiyongere bugaragara bwibibabi numubare. Bivugwa ko Benzyladenine yongerera umusaruro amababi ya calla15. Ukurikije ibyavuye muri ubu bushakashatsi, uburyo bwose bwo kuvura bwateje imbere amababi n'umubare. Acide Gibberellic + benzyladenine niyo miti ivura neza kandi bivamo umubare munini nubuso bwamababi. Iyo ukura dwarf schefflera mumazu, hashobora kubaho kwiyongera kugaragara mumibare yamababi.
Ubuvuzi bwa GA3 bwongereye interode ugereranije na benzyladenine (BA) cyangwa nta kuvura imisemburo. Igisubizo kirumvikana ukurikije uruhare rwa GA mugutezimbere iterambere7. Gukura kw'ibiti nabyo byagaragaje ibisubizo bisa. Acide ya Gibberellic yongereye uburebure bwuruti ariko igabanya diameter. Ariko, gushyira hamwe kwa BA na GA3 byongereye cyane uburebure bwuruti. Ubu bwiyongere bwari hejuru ugereranije nibihingwa bivurwa na BA cyangwa bidafite imisemburo. Nubwo aside ya gibberellic na cytokinine (CK) muri rusange biteza imbere imikurire yibihingwa, rimwe na rimwe bigira ingaruka zinyuranye mubikorwa bitandukanye35. Kurugero, imikoranire mibi yagaragaye mukwiyongera kwuburebure bwa hypocotyl mubihingwa bivurwa na GA na BA36. Kurundi ruhande, BA yiyongereye cyane ingano yumuzi (Imbonerahamwe 1). Ubwiyongere bwumuzi bitewe na BA exogenous BA byagaragaye mubimera byinshi (urugero ubwoko bwa Dendrobium na Orchide) 37,38.
Imiti yose ivura imisemburo yongereye umubare wamababi mashya. Ubwiyongere busanzwe bwibibabi hamwe nuburebure bwuruti binyuze mubuvuzi bukenewe ni ubucuruzi. Umubare wamababi mashya nikimenyetso cyingenzi cyo gukura kwibimera. Gukoresha imisemburo ya exogenous ntabwo yakoreshejwe mubikorwa byubucuruzi bya Liriodendron tulipifera. Nyamara, ingaruka ziterambere ryiterambere rya GA na CK, zikoreshwa muburinganire, zishobora gutanga ubumenyi bushya mugutezimbere ubuhinzi bwiki gihingwa. Ikigaragara ni uko ingaruka zo kuvura BA + GA3 zivuye hejuru kuruta iya GA cyangwa BA yatanzwe wenyine. Acide ya Gibberellic yongera umubare wamababi mashya. Mugihe amababi mashya akura, kongera umubare wamababi mashya birashobora kugabanya imikurire yamababi39. GA yavuzwe ko izamura ubwikorezi bwa sucrose kuva mu mwobo kugera mu ngingo 40,41. Byongeye kandi, gukoresha GA mu buryo budasanzwe ku bimera bimaze imyaka myinshi bishobora guteza imbere imikurire y’ibimera nkamababi n’imizi, bityo bikarinda guhinduka kuva mu bimera kugera ku mikurire y’imyororokere42.
Ingaruka za GA mukwongera ibimera byumye bishobora gusobanurwa no kwiyongera kwa fotosintezeza kubera kwiyongera kwamababi43. GA yavuzwe ko itera ubwiyongere bwibibabi bya Maize34. Ibisubizo byerekanaga ko kongera BA yibanze kuri 200 mg / L bishobora kongera uburebure numubare wamashami yisumbuye hamwe nubunini bwumuzi. Acide ya Gibberellique igira ingaruka kumikorere ya selile nko gukurura ingirabuzimafatizo no kuramba, bityo bigatuma imikurire ikura43. Byongeye kandi, HA yagura urukuta rw'utugingo ngengabuzima ya hydrolyzing mu isukari, bityo igabanya ubushobozi bw'amazi y'akagari, bigatuma amazi yinjira mu kagari kandi amaherezo biganisha ku kurambura selile44.

 


Igihe cyo kohereza: Jun-11-2024