Ubu bushakashatsi bwerekana ko fungus Kosakonia oryziphila NP19 ifitanye isano n'imizi, yakuwe mu mizi y'umuceri, ari umuti wica udukoko n'ibinyabutabire bitera ingaruka mbi ku bimera mu kugenzura blast y'umuceri. Igerageza ryakozwe mu buryo bwa vitro ku mababi mashya y'imbuto z'umuceri za Khao Dawk Mali 105 (KDML105). Ibisubizo byagaragaje ko NP19 yabujije neza kumera kwa conidia y'umuceri. Kwandura indwara z'ibihumyo byakumiriwe mu buryo butatu butandukanye bwo kuvura: gutera umuceri na NP19 na conidia y'ibihumyo; gutera amababi icyarimwe na NP19 na conidia y'ibihumyo; no gutera amababi na conidia y'ibihumyo hagakurikiraho kuvurwa na NP19 nyuma y'amasaha 30. Byongeye kandi, NP19 yagabanyije gukura kwa hyphal y'ibihumyo ku kigero cya 9.9–53.4%. Mu igerageza ry’inkono, NP19 yongereye ibikorwa bya peroxidase (POD) na superoxide dismutase (SOD) ku kigero cya 6.1% kugeza kuri 63.0% na 3.0% kugeza kuri 67.7%, uko bikurikirana, bigaragaza uburyo bwo kwirwanaho kw’ibimera. Ugereranyije n’ibipimo bya NP19 bitanduye, ibimera by’umuceri byanduye NP19 byagaragaje ubwiyongere bw’ibipimo bya pigment ku kigero cya 0.3%–24.7%, umubare w’ibinyampeke byuzuye kuri panicle kuri 4.1%, umusaruro w’ibinyampeke byuzuye kuri 26.3%, igipimo cy’umusaruro ku kigero cya 34.4%, n’umubare w’ibinyampeke bya aromatic 2-acetyl-1-pyrroline (2AP) ku kigero cya 10.1%. Mu bimera by’umuceri byanduye NP19 na blast, ukwiyongera kwabyo kwari 0.2%–49.2%, 4.6%, 9.1%, 54.4%, na 7.5%, uko bikurikirana. Ubushakashatsi bwo mu murima bwagaragaje ko ibihingwa by'umuceri byakuwemo cyangwa byatewe NP19 byagaragaje ukwiyongera k'umubare w'ibinyampeke byuzuye kuri panicle kuri 15.1–27.2%, umusaruro wuzuye ku binyampeke kuri 103.6–119.8%, n'ingano ya 2AP kuri 18.0–35.8%. Ibi bihingwa by'umuceri nabyo byagaragaje imikorere ya SOD iri hejuru (6.9–29.5%) ugereranije n'ibihingwa by'umuceri byanduye bitatewe NP19. Gushyira NP19 ku biti nyuma yo kwandura byagabanyije iterambere ry'ibisebe. Bityo, K. oryphila NP19 byagaragaye ko ari umuti ushobora gukura w'ibimera utera imbaraga zo kurwanya ibisebe no kwica udukoko mu kurwanya ibisebe.
Ariko, akamaro k'imiti yica udukoko gaterwa n'ibintu byinshi, birimo uburyo ikoreshwa, igihe n'uburyo ikoreshwa, ubukana bw'indwara, uburyo bwo guhanura indwara, ndetse n'ubwoko bw'imiti irwanya udukoko. Byongeye kandi, gukoresha imiti yica udukoko bishobora gutera uburozi busigaye mu bidukikije no guteza akaga ku buzima bw'abayikoresha.
Mu igerageza ryakozwe mu nkono, imbuto z'umuceri zaragizwe umuti wica udukoko ku buso hanyuma zimera nk'uko byasobanuwe haruguru. Hanyuma zatewemo K. oryphila NP19 hanyuma zishyirwa mu masahani y'imiti. Ingemwe zashyizwe mu nkono mu gihe cy'iminsi 30 kugira ngo ingemwe z'umuceri zishobore kumera. Ingemwe zashyizwe mu nkono. Mu gihe cyo gutera, ibihingwa by'umuceri byashyizwemo ifumbire kugira ngo bitegure kwandura ibihumyo bitera umuceri guturika no kugira ngo harebwe uburyo bidashobora kwihanganira.
Mu igerageza ryakozwe mu murima, imbuto zatewe zandujwe na Aspergillus oryzae NP19 zavuwe hakoreshejwe uburyo bwavuzwe haruguru kandi zigaburwamo amatsinda abiri: imbuto zandujwe na Aspergillus oryzae NP19 (RS) n'imbuto zitandujwe (US). Imbuto zatewe zatewe mu masahani arimo ubutaka bwasizwe umuti (uruvange rw'ubutaka, igishishwa cy'umuceri cyatwitswe, n'ifumbire y'imborera ku gipimo cya 7:2:1 ukurikije uburemere) maze zishyirwa mu mazuru mu gihe cy'iminsi 30.
Oryziphila conidial suspension yongewe ku muceri wa R, hanyuma nyuma y'amasaha 30 yo guteka, hongewemo 2 μl ya K. oryziphila NP19 ahantu hamwe. Amafunguro yose ya Petri yashyizwe ku bushyuhe bwa 25°C mu mwijima mu gihe cy'amasaha 30 hanyuma ashyirwa ku rumuri rudasibangana. Buri tsinda ryasubiwemo inshuro eshatu. Nyuma y'amasaha 72 yo guteka, ibice by'ibimera byasuzumwe kandi bikorerwa microscopy ya electron. Muri make, ibice by'ibimera byashyizwe mu munyu wa fosfeti urimo 2.5% (v/v) glutaraldehyde hanyuma bikurwamo amazi mu buryo butandukanye bwa ethanol. Ingero zarumishijwe na dioxyde de carbone, hanyuma zishyirwa ku muriro wa zahabu hanyuma zirebwa munsi ya microscope ya electron ya scanning mu gihe cy'iminota 15.
Igihe cyo kohereza: Ukwakira 13-2025