Paclobutrazol itera gukora triterpenoid biosynthesis igabanya uburyo bwo guhindura transcription SlMYB mu buvuzi bw'ibimera byo mu Buyapani.

Ibihumyo binini bifite ubwoko bwinshi kandi butandukanye bwa metabolites zigira uruhare runini mu buzima kandi bifatwa nk'umutungo kamere w'agaciro. Phellinus igniarius ni igihumyo kinini gikoreshwa mu buryo busanzwe haba mu buvuzi no mu biribwa, ariko ishyirwa ryacyo mu byiciro n'izina ryacyo ry'ikilatini biracyari impaka. Bakoresheje isesengura ry'imiterere y'uturemangingo twinshi, abashakashatsi bemeje ko Phellinus igniarius n'ubwoko busa nabyo biri mu bwoko bushya bw'ubwoko maze bashyiraho ubwoko bwa Sanghuangporus. Igihumyo cya honeysuckle Sanghuangporus lonicericola ni kimwe mu bwoko bwa Sanghuangporus bwamenyekanye ku isi yose. Phellinus igniarius yakuruye abantu benshi kubera imiterere yacyo itandukanye mu buvuzi, harimo polysaccharides, polyphenols, terpenes, na flavonoids. Triterpenes ni byo bintu by'ingenzi bikora mu buvuzi muri ubu bwoko, bigaragaza ibikorwa byo kurwanya antioxidant, bagiteri, no kurwanya kanseri.
Triterpenoids zifite ubushobozi bukomeye bwo gukoreshwa mu bucuruzi. Bitewe n'ubuke bw'umutungo kamere wa Sanghuangporus, kongera imikorere myiza n'umusaruro wayo ni ingenzi cyane. Muri iki gihe, hari intambwe yatewe mu kongera umusaruro w'ibintu bitandukanye bya Sanghuangporus hakoreshejwe imiti itera imbaraga kugira ngo igenzure ingamba zo guhinga mu mazi. Urugero, aside polyunsaturated fatty, fungal exitors11 na phytohormones (harimo methyl jasmonate na aside salicylic14) byagaragaye ko byongera umusaruro wa triterpenoid muri Sanghuangporus. Abashinzwe kugenzura imikurire y'ibimera(Amatsinda y'ibyiciro (PGRs))ishobora kugenzura uburyo ibimera bikura mu buryo bwa metabolite y’inyongera mu bimera. Muri ubu bushakashatsi, PBZ, ishami rigenzura imikurire y’ibimera rikoreshwa cyane mu kugenzura imikurire y’ibimera, umusaruro, ubwiza n’imiterere y’umubiri, ryakoreweho ubushakashatsi. By’umwihariko, ikoreshwa rya PBZ rishobora kugira ingaruka ku nzira ya terpenoid biosynthetic mu bimera. Guhuza gibberellins na PBZ byongereye quinone methide triterpene (QT) muri Montevidia floribunda. Imiterere ya terpenoid y’amavuta ya lavender yahinduwe nyuma yo kuvurwa na 400 ppm PBZ. Ariko, nta raporo zihari ku ikoreshwa rya PBZ ku bihumyo.
Uretse ubushakashatsi bwibanda ku kwiyongera k'umusaruro wa triterpene, hari ubushakashatsi bwagaragaje uburyo bwo kugenzura triterpene biosynthesis muri Moriformis hakoreshejwe imiti itera ibinyabutabire. Muri iki gihe, ubushakashatsi burimo kwibanda ku ihinduka ry'urwego rw'imikorere ya genes z'imiterere zijyanye na triterpene biosynthesis mu nzira ya MVA, bigatuma umusaruro wa terpenoid wiyongera.12,14 Ariko, inzira zishingiye kuri izi genes zizwi, cyane cyane ibintu byo kwandika bigenga imikorere yazo, ntizisobanutse neza mu buryo bwo kugenzura triterpene biosynthesis muri Moriformis.
Muri ubu bushakashatsi, hakozwe ubushakashatsi ku ngaruka zitandukanye z’ibipimo by’imikurire y’ibimera (PGRs) ku musaruro wa triterpene no gukura kwa mycelial mu gihe cyo guhinga honeysuckle (S. lonicericola) mu mazi. Nyuma yaho, metabolomics na transcriptomics byakoreshejwe mu gusesengura imiterere ya triterpene n’imiterere y’uturemangingo tw’uturemangingo twifashishwa mu gukora triterpene mu gihe cyo kuvurwa PBZ. Amakuru ya RNA-sequencing na bioinformatics yagaragaje uburyo bwo kwandika MYB (SlMYB). Byongeye kandi, hakozwe mutants kugira ngo hemezwe ingaruka zo kugenzura za gene ya SlMYB ku gukora triterpene no kumenya gene zishobora kuba zihari. Isuzuma rya electrophoretic mobility shift assays (EMSA) ryakoreshejwe mu kwemeza imikoranire ya poroteyine ya SlMYB n’abatera inkunga gene za SlMYB. Muri make, intego y'ubu bushakashatsi kwari ugukangura uburyo bwo gukora triterpene hifashishijwe PBZ no kumenya MYB transcription factor (SlMYB) igenga mu buryo butaziguye imiterere ya triterpene biosynthesis irimo MVD, IDI, na FDPS muri S. lonicericola mu gusubiza PBZ induction.
Gutera IAA na PBZ byazamuye cyane umusaruro wa triterpenoid muri honeysuckle, ariko ingaruka za PBZ zo gutera zari nziza cyane. Kubwibyo, PBZ yagaragaye ko ari yo itera imbere kurusha izindi ku gipimo cya 100 mg/L, ibi bikaba bikwiye kwigwaho cyane.