Paclobutrazol itera triterpenoid biosynthesis muguhagarika imiyoboro mibi ya SlMYB mibi mu Buyapani.

Ibihumyo binini bifite ubutunzi butandukanye kandi butandukanye bwa bioactive metabolite kandi bifatwa nkibinyabuzima bifite agaciro. Phellinus igniarius ni ibihumyo binini bisanzwe bikoreshwa mu rwego rwo kuvura no kurya, ariko ibyiciro byayo n'izina ry'ikilatini bikomeje kutavugwaho rumwe. Bakoresheje isesengura rihuza ibice byinshi, abashakashatsi bemeje ko Phellinus igniarius n’ibindi bisa bisa n’ubwoko bushya kandi bashiraho ubwoko bwa Sanghuangporus. Ibihumyo bya Honeysuckle Sanghuangporus lonicericola ni bumwe mu bwoko bwa Sanghuangporus bwamenyekanye ku isi. Phellinus igniarius yakunze kwitabwaho cyane kubera imiti itandukanye y’imiti, harimo polysaccharide, polifenol, terpène, na flavonoide. Triterpène nurufunguzo rwimiti ikora muri ubu bwoko, rugaragaza antioxydants, antibacterial, na antitumor.
Triterpenoide ifite amahirwe menshi yo gukoresha ubucuruzi. Bitewe nuko bidakunze kuboneka umutungo wa Sanghuangporus wo muri gasozi, kuzamura neza imikorere ya biosintetike n'umusaruro bifite akamaro kanini. Kugeza ubu, hari intambwe imaze guterwa mu kuzamura umusaruro wa metabolite itandukanye ya kabiri ya Sanghuangporus ukoresheje imashini itera imiti kugira ngo igenzure ingamba zo gusembura amazi. Kurugero, polyunsaturated fatty acide, elicitor ya fungal11 na phytohormone (harimo na methyl jasmonate na acide salicylic14) byagaragaye ko byongera umusaruro wa triterpenoid muri Sanghuangporus. Igenzura ryikura ryibihingwa(PGR)irashobora kugenga biosynthesis ya metabolite ya kabiri mubihingwa. Muri ubu bushakashatsi, ubushakashatsi bwakozwe na PBZ, bugenzura imikurire y’ibihingwa bukoreshwa cyane mu kugenzura imikurire y’ibihingwa, umusaruro, ubwiza n’imiterere ya physiologiya. By'umwihariko, gukoresha PBZ birashobora kugira ingaruka kuri terpenoid biosynthetic inzira mubihingwa. Guhuza gibberelline na PBZ byongereye quinone methide triterpene (QT) muri Montevidia floribunda.Ibigize inzira ya terpenoid yinzira yamavuta ya lavender yahinduwe nyuma yo kuvurwa hamwe na 400 ppm PBZ. Ariko, nta raporo zerekeye ikoreshwa rya PBZ ku bihumyo.
Usibye ubushakashatsi bwibanze ku kwiyongera k'umusaruro wa triterpene, ubushakashatsi bumwe na bumwe bwanasobanuye uburyo bwo kugenzura imikorere ya triterpene biosynthesis muri Moriformis hifashishijwe imiti itera imiti. Kugeza ubu, ubushakashatsi bwibanze ku guhindura urwego rwerekana imiterere yimiterere yimiterere ijyanye na triterpene biosynthesis mu nzira ya MVA, biganisha ku kwiyongera k'umusaruro wa terpenoid.12,14 Icyakora, inzira zishingiye kuri genes zizwi zizwi cyane cyane ibintu byandikirana zigenga imvugo zazo, ntizisobanutse neza muburyo bwo kugenzura triterpene biosynthesis muri Moriformis.
Muri ubu bushakashatsi, hasuzumwe ingaruka ziterwa n’ubushakashatsi butandukanye bwo gukura kw'ibimera (PGRs) ku musaruro wa triterpene no gukura kwa myisiyeli mu gihe cyo gusembura honeysuckle (S. lonicericola). Nyuma yaho, metabolomics na transcriptomics byakoreshejwe mu gusesengura imiterere ya triterpene nuburyo bwo kwerekana imiterere ya gene igira uruhare muri triterpene biosynthesis mugihe cyo kuvura PBZ. RNA ikurikirana hamwe na bioinformatics yamakuru yongeye kwerekana intego yo kwandukura intego ya MYB (SlMYB). Byongeye kandi, mutant zakozwe kugirango zemeze ingaruka za gene ya SlMYB kuri triterpene biosynthesis no kumenya genes zishobora kubaho. Electrophoretic mobile mobile shift assays (EMSA) yakoreshejwe kugirango hemezwe imikoranire ya poroteyine ya SlMYB hamwe na porotokoro ya gen intego ya SlMYB. Muri make, intego yubu bushakashatsi kwari ugukangurira triterpene biosynthesis ukoresheje PBZ no kumenya ibintu byandikirwa MYB (SlMYB) bigenga byimazeyo genoside ya triterpene harimo MVD, IDI, na FDPS muri S. lonicericola mugusubiza kwinjiza PBZ.
Kwinjiza byombi IAA na PBZ byongereye cyane umusaruro wa triterpenoid muri honeysuckle, ariko ingaruka zo kwinjiza PBZ zagaragaye cyane. Kubwibyo, PBZ wasangaga aribwo buryo bwiza cyane bwiyongera kuri 100 mg / L, bukwiye ubushakashatsi.