Fosifora ikora igenzura rikura ryikura rya DELLA muri Arabidopsis iteza imbere ishyirahamwe rya histone H2A na chromatine.

Intungamubiri za DELLA zabitswe nezaabashinzwe iteramberebigira uruhare runini mukugenzura iterambere ryibimera hasubijwe imbere nibidukikije. DELLA ikora nk'umugenzuzi w'inyandiko mvugo kandi yinjizwa mubateza imbere intego yo guhuza ibintu (TFs) na histone H2A binyuze muri domaine yayo GRAS. Ubushakashatsi buherutse gukorwa bwerekanye ko DELLA itajegajega igenzurwa nyuma yubuhinduzi hakoreshejwe uburyo bubiri: polyubiquitination iterwa na phytohormone gibberellin, iganisha ku kwangirika kwayo vuba, hamwe no guhuza uduce duto duto dusa na ubiquitin (SUMO) kugirango twongere ubwinshi bwayo. Mubyongeyeho, ibikorwa bya DELLA bigengwa ningaruka ebyiri na glycosylations zitandukanye: imikoranire ya DELLA-TF yongerewe imbaraga na O-fucosylation ariko ikabuzwa na O-ihuza N-acetylglucosamine (O-GlcNAc). Icyakora, uruhare rwa fosifora ya DELLA ntirurasobanuka neza, kubera ko ubushakashatsi bwibanze bwerekanye ibisubizo bivuguruzanya, uhereye ku byerekana ko fosifora itera cyangwa igabanya iyangirika rya DELLA ku bandi byerekana ko fosifora itagira ingaruka ku ituze ryayo. Hano, tumenye imbuga za fosifora muri REPRESSORga1-3. Ishyirahamwe rya RGA hamwe nabateza imbere intego. Ikigaragara ni uko fosifora itagira ingaruka ku mikoranire ya RGA-TF cyangwa ihame rya RGA. Ubushakashatsi bwacu bugaragaza uburyo bwa molekuline ikoreshwa na fosifora itera ibikorwa bya DELLA.
Kugirango usobanure uruhare rwa fosifora mugutunganya imikorere ya DELLA, ni ngombwa kumenya ibibanza bya fosifora ya DELLA muri vivo no gukora isesengura ryimikorere mubihingwa. Mugihe cyo kweza ibimera bivamo ibihingwa bikurikirwa nisesengura rya MS / MS, twabonye fosifosite nyinshi muri RGA. Mubihe byo kubura GA, fosifora ya RHA iriyongera, ariko fosifora ntiguhindura ituze. Icy'ingenzi, hamwe na IP hamwe na ChIP-qPCR byagaragaje ko fosifora mu karere ka PolyS / T mu karere ka RGA iteza imbere imikoranire yayo na H2A no gufatanya n’abateza imbere intego, bikagaragaza uburyo fosifora itera imikorere ya RGA.
RGA yashakishijwe kugirango yibasire chromatine binyuze mumikoranire ya subdomain ya LHR1 na TF hanyuma ihuza H2A ibinyujije mukarere kayo ka PolyS / T hamwe na PFYRE, ikora H2A-RGA-TF kugirango ihoshe RGA. Fosifora ya Pep 2 mukarere ka PolyS / T hagati ya DELLA nubutegetsi bwa GRAS na kinase itazwi byongera RGA-H2A guhuza. Poroteyine ya rgam2A ikuraho fosifora ya RGA kandi igahindura poroteyine itandukanye kugirango ibangamire guhuza H2A. Ibi bivamo guhungabanya imikoranire yigihe gito TF-rgam2A no gutandukana kwa rgam2A kuva chromatine. Iyi shusho yerekana gusa gukandamizwa kwandikirwa RGA-yunganirwa. Uburyo busa bushobora gusobanurwa kubikorwa bya RGA-byahujwe no gukora transcriptional transcription, usibye ko ikigo cya H2A-RGA-TF cyateza imbere iyandikwa rya gene hamwe na dephosifora ya rgam2A byagabanya kwandukura. Igishushanyo cyahinduwe kuva Huang n'abandi.21.
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Ibyinshi muri proteomics ya proteomics yatanzwe muri Consortium ya ProteomeXchange binyuze mububiko bwabafatanyabikorwa PRIDE66 hamwe na dataset iranga PXD046004. Andi makuru yose yabonetse muri ubu bushakashatsi atangwa mu makuru yinyongera, amadosiye yinyongera, hamwe namakuru yububiko. Inkomoko yamakuru yatanzwe kuriyi ngingo.