Fosforiyumu ikora ku buryo DELLA ikora ku ireme ry’imikurire y’ibinyabuzima muri Arabidopsis binyuze mu guteza imbere isano iri hagati ya histone H2A na chromatin.

Poroteyine za DELLA ni ingenzi mu kubikaabashinzwe kugenzura iterambereIbyo bigira uruhare runini mu kugenzura iterambere ry'ibimera bitewe n'ibimenyetso by'imbere mu mubiri n'ibidukikije. DELLA ikora nk'umugenzuzi w'inyandiko kandi ishyirwa mu bashinzwe kumenyekanisha ibikorwa binyuze mu guhuza ibintu bya transcription (TFs) na histone H2A binyuze mu gice cyayo cya GRAS. Ubushakashatsi buherutse bwerekanye ko DELLA ihamye nyuma yo guhindura ibintu ikoresheje uburyo bubiri: polyubiquitination iterwa na phytohormone gibberellin, bigatuma igabanuka vuba, no guhuza small ubiquitin-like modifiers (SUMO) kugira ngo yongere ubwiyongere bwayo. Byongeye kandi, imikorere ya DELLA igenzurwa mu buryo bubiri butandukanye na glycosylations: DELLA-TF ikorana neza na O-fucosylation ariko ikabuzwa na O-linked N-acetylglucosamine (O-GlcNAc) modification. Ariko, uruhare rwa DELLA phosphorylation ntirurasobanuka neza, kuko ubushakashatsi bwabanje bwagaragaje ibisubizo bivuguruzanya, uhereye ku byagaragaje ko phosphorylation itera cyangwa igagabanya iyangirika rya DELLA kugeza ku bindi bigaragaza ko phosphorylation idagira ingaruka ku ituze ryayo. Aha, turagaragaza ahantu phosphorylation muri REPRESSORga1-3(RGA, AtDELLA) yakuwe muri Arabidopsis thaliana hakoreshejwe isesengura rya mass spectrometry kandi igaragaza ko phosphorylation ya peptide ebyiri za RGA mu turere twa PolyS na PolyS/T itera H2A gufatana no kongera imikorere ya RGA. Ihuriro rya RGA n'ibikoresho bigamije gutera inkunga. Ikigaragara ni uko phosphorylation idakora ku mikoranire ya RGA-TF cyangwa ku buryo RGA idahindagurika. Ubushakashatsi bwacu bwagaragaje uburyo molekile ikoresha phosphorylation itera imikorere ya DELLA.
Kugira ngo dusobanukirwe uruhare rwa fosforili mu kugenzura imikorere ya DELLA, ni ngombwa kumenya ahantu DELLA ikorera mu mubiri no gukora isesengura ry’imikorere mu bimera. Mu gusukura ibikomoka ku bimera bikurikirwa no gusesengura MS/MS, twabonye fosforili nyinshi muri RGA. Mu gihe cy’ibura rya GA, fosforilili ya RHA iriyongera, ariko fosforilili ntigira ingaruka ku buryo igumaho. Icy'ingenzi ni uko isuzuma rya co-IP na ChIP-qPCR ryagaragaje ko fosforili ...
RGA ikoreshwa mu gushaka chromatin binyuze mu mikoranire y’agace ka LHR1 na TF hanyuma igafatana na H2A binyuze mu gace kayo ka PolyS/T na PFYRE, igakora H2A-RGA-TF complex kugira ngo ihamye RGA. Phosphorylation ya Pep 2 mu gace ka PolyS/T hagati ya DELLA domaine na GRAS domaine hakoreshejwe kinase itazwi yongera uburyo bwa RGA-H2A. Poroteyine ya rgam2A mutant ikuraho phosphorylation ya RGA kandi igakoresha imiterere itandukanye ya poroteyine kugira ngo ivange H2A link. Ibi bituma habaho kudahuza kw’imikorere ya TF-rgam2A y’igihe gito no kwitandukanya kwa rgam2A na chromatin y’icyitegererezo. Iyi shusho igaragaza gusa RGA-mediated transcriptional repression. Uburyo nk’ubwo bushobora gusobanurwa ku gukora transcriptional ...
Amakuru yose yerekeye imibare yasesenguwe hakoreshejwe Excel, kandi itandukaniro rikomeye ryagaragaye hakoreshejwe ikizamini cya t cy’umunyeshuri. Nta buryo bw’ibarurishamibare bwakoreshejwe kugira ngo hamenyekane ingano y’icyitegererezo mbere na mbere. Nta makuru yakuwe mu isesengura; igerageza ntiryakozwe ku buryo butunguranye; abashakashatsi ntibari impumyi ku ikwirakwizwa ry’amakuru mu gihe cy’igerageza no gusuzuma ibyavuye mu bushakashatsi. Ingano y’icyitegererezo igaragazwa mu nkuru y’ishusho n’inyandiko y’aho amakuru yaturutse.
Kugira ngo umenye byinshi ku bijyanye n'igishushanyo mbonera cy'ubushakashatsi, reba Incamake ya Raporo y'Ubushakashatsi bw'Ibintu Bisanzwe ijyanye n'iyi nkuru.
Amakuru ya proteomics ya Mass spectrometry yatanzwe ku itsinda rya ProteomeXchange binyuze mu bubiko bw'abafatanyabikorwa ba PRIDE66 bufite indangamuntu ya data PXD046004. Andi makuru yose yabonetse muri ubu bushakashatsi agaragazwa mu makuru y'inyongera, dosiye z'amakuru y'inyongera, na dosiye z'amakuru y'umwimerere. Amakuru y'inkomoko aratangwa kuri iyi nkuru.