Poroteyine za DELLA zirabikwaabashinzwe kugenzura iteramberebigira uruhare runini mu iterambere ry'ibimera bitewe n'ibimenyetso by'imbere n'inyuma. Nk'abagenzura uburyo bwo kwandika, DELLA zifatanya n'ibintu bya transcription (TFs) na histone H2A binyuze mu nzego zabo za GRAS kandi zigashakwa kugira icyo zikora ku bateza imbere. Ubushakashatsi buherutse bwerekanye ko DELLA ihamye igenzurwa nyuma yo guhindura ibintu hakoreshejwe uburyo bubiri: polyubiquitination iterwa na hormone y'ibimera.gibberellin, ibyo bigatuma zisenyuka vuba, kandi zigahuza na small ubiquitin-like modifier (SUMO), ibyo byongera ubwiyongere bwazo. Byongeye kandi, imikorere ya DELLA igenzurwa mu buryo bubiri butandukanye bwa glycosylation: O-fucosylation yongera imikoranire ya DELLA-TF, mu gihe O-linked N-acetylglucosamine (O-GlcNAc) ibuza imikoranire ya DELLA-TF. Ariko, uruhare rwa DELLA phosphorylation ntirurasobanuka neza kuko ubushakashatsi bwabanje bwagaragaje ibisubizo bivuguruzanya, aho bamwe bavuga ko phosphorylation itera cyangwa igahagarika iyangirika rya DELLA naho abandi bakavuga ko phosphorylation idahindura imiterere yazo. Aha, turagaragaza ahantu ha phosphorylation muri GA1-3 repressor (RGA), AtDELLA, yakuwe muri Arabidopsis thaliana hakoreshejwe mass spectrometry kandi igaragaza ko phosphorylation ya peptides ebyiri za RGA mu turere twa PolyS na PolyS/T yongera imikorere ya RGA binyuze mu guteza imbere H2A bihuza kandi RGA ihurirana n'abaterankunga. Ikigaragara ni uko phosphorylation itagize ingaruka ku mikoranire ya RGA-TF cyangwa ku ituze rya RGA. Ubushakashatsi bwacu bwagaragaje uburyo molekile ikoresha phosphorylation itera DELLA gukora.
Isesengura ryacu rya spectrometric ryagaragaje ko Pep1 na Pep2 byombi byari bifite fosfori nyinshi muri RGA mu gihe Ga1 yari idafite GA. Uretse ubu bushakashatsi, ubushakashatsi bwa phosphoproteomic bwagaragaje kandi fosfori ya Pep1 muri RGA, nubwo uruhare rwayo rutarakorwa53,54,55. Mu buryo bunyuranye, fosfori ya Pep2 ntiyigeze isobanurwa mbere kuko iyi peptide yashoboraga kuboneka gusa hakoreshejwe transgene ya RGAGKG. Nubwo mutation ya m1A, yakuyeho fosfori ya Pep1, yagabanyije gato ibikorwa bya RGA muri planta, yagize ingaruka zo kongeramo iyo ihujwe na m2A mu kugabanya ibikorwa bya RGA (Ishusho y'inyongera ya 6). Icy'ingenzi ni uko fosfori ya Pep1 yagabanutse cyane muri sly1 mutant ya GA ugereranije na ga1, bigaragaza ko GA itera imbere dephosphorylation ya RGA, ikagabanya ibikorwa byayo. Uburyo GA ikoresha mu guhagarika fosfori ya RGA busaba iperereza ryinshi. Kimwe mu bishoboka ni uko ibi bigerwaho binyuze mu kugenzura poroteyine kinase itazwi. Nubwo ubushakashatsi bwagaragaje ko imikorere ya poroteyine kinase ya CK1 EL1 igabanuka bitewe na GA mu muceri41, ibisubizo byacu bigaragaza ko impinduka zo mu rwego rwo hejuru za Arabidopsis EL1 homologue (AEL1-4) zidagabanya phosphorylation ya RGA. Dukurikije ibyavuye mu bushakashatsi bwacu, inyigo iherutse gukorwa kuri phosphoproteomic ikoresheje imirongo ya Arabidopsis AEL isohora cyane hamwe na ael triple mutant ntabwo yagaragaje poroteyine za DELLA nk'ibice by'izi kinase56. Ubwo twateguraga inyandiko, byatangajwe ko GSK3, gene ikora GSK3/SHAGGY-like kinase mu ngano (Triticum aestivum), ishobora phosphorylate DELLA (Rht-B1b)57, nubwo phosphorylation ya Rht-B1b na GSK3 itaremezwa muri planta. Impinduka za enzymatic muri vitro imbere ya GSK3 zigakurikirwa n'isesengura rya mass spectrometry ryagaragaje ahantu hatatu ha phosphorylation hagati ya DELLA na GRAS domains za Rht-B1b (Ishusho y'inyongera ya 3). Gusimbuza serine na alanine ahantu hose hatatu ha phosphorylation byatumye imikorere ya Rht-B1b igabanuka mu ngano ya transgenic, nk'uko twabibonye ko gusimbuza alanine muri Pep2 RGA byagabanyije imikorere ya RGA. Ariko, isuzuma rya poroteyine muri vitro ryagaragaje ko phosphorylation ishobora no gutuma Rht-B1b57 ihagarara neza. Ibi bitandukanye n'ibyavuye mu bushakashatsi bwacu bigaragaza ko gusimbuza alanine muri Pep2 RGA bidahindura imikorere yayo mu bimera. GSK3 mu ngano ni imiterere ya poroteyine 2 idakoresha brassinosteroid (BIN2) muri Arabidopsis 57. BIN2 ni umugenzuzi mubi w’ibimenyetso bya BR, kandi BR ikora inzira yayo y’ibimenyetso itera kwangirika kwa BIN2 58. Twagaragaje ko kuvura BR bidagabanya uburinganire bwa RGA 59 cyangwa urugero rwa phosphorylation muri Arabidopsis (Ishusho y’inyongera ya 2), bigaragaza ko RGA idashobora kuba ya phosphorylation na BIN2.
Amakuru yose yerekeye imibare yasesenguwe hakoreshejwe Excel, kandi itandukaniro rikomeye ryagaragaye hakoreshejwe ikizamini cya t cy’umunyeshuri. Nta buryo bw’ibarurishamibare bwakoreshejwe kugira ngo hamenyekane mbere y’igihe ingano y’ingero. Nta makuru yakuwe mu isesengura; igerageza ntiryakozwe ku buryo butunguranye; kandi abashakashatsi bari bazi uburyo bwo gutanga amakuru mu gihe cy’igerageza n’isuzuma ry’ibyavuye mu bushakashatsi. Ingano z’ingero zitangwa mu nkuru z’imibare no mu madosiye y’amakuru fatizo.
Igihe cyo kohereza: 15 Mata 2025