Abashakashatsi bavumbuye uburyo ibimera bigenzura poroteyine za DELLA.

Abashakashatsi bo mu Ishami rya Biochimie mu Ishuri Rikuru ry’Ubumenyi ry’Abahinde (IISc) bavumbuye uburyo bwari bumaze igihe kirekire bushakishwamu kugenzura imikurire y'ibimera byo ku butaka bya kera nka bryophytes (itsinda ririmo mosses na liverworts) byagumanye mu bimera byaje kurabya nyuma.
Ubushakashatsi bwasohotse mu kinyamakuru Nature Chemical Biology, bwibanze ku kugenzura poroteyine za DELLA, ikigo cy’ingenzi gishinzwe gukura kw’uturemangingo, kigabanya igabanyuka ry’uturemangingo mu turemangingo tw’inda (embryophytes).
Igishimishije ni uko bryophytes, ibimera bya mbere byagaragaye ku butaka mu myaka igera kuri miliyoni 500 ishize, bitagira receptor ya GID1, nubwo byakoraga phytohormone GA. Ibi bitera kwibaza uburyo imikurire n'iterambere ry'ibi bimera byo ku butaka bya mbere byagenzurwaga.
Bakoresheje liverwort Marchantia polymorpha nk'icyitegererezo cy'uburyo, abashakashatsi basanze ibi bimera bya kera bikoresha enzyme yihariye, MpVIH, ikora inositol pyrophosphate (InsP₈), kugira ngo isenye DELLA nta ruhare rwayo.aside gibberellic.
Abashakashatsi bakoresheje sisitemu ya CRISPR-Cas9 kugira ngo bakureho gene ikora kuri enzyme ya VIH, byemeza uruhare rwayo. Ibimera bidafite VIH ikora byagaragaje inenge zikomeye mu mikurire n'imiterere idahwitse, nk'amababi magufi, gukura nabi kw'imirasire, no kubura calyxes. Izi nenge zakuweho no guhindura genome y'ibimera kugira ngo habeho impera imwe gusa (N-terminus) ya enzyme ya VIH. Bakoresheje ubuhanga buhanitse bwa chromatography, itsinda ryavumbuye ko N-terminus irimo domaine ya kinase itera imbaraga mu gukora InsP₈.
Abashakashatsi bavumbuye ko DELLA ari imwe mu ntego z'uturemangingo twa VIH kinase. Byongeye kandi, babonye ko imiterere y'ibimera bidafite MpVIH isa n'iy'ibimera bya M. polymorpha hamwe n'ubwiyongere bwa DELLA.
“Kuri iyi ngingo, twari twishimiye gusobanukirwa niba iterambere rya DELLA cyangwa imikorere yaryo iri kwiyongera mu bimera bidafite MpVIH,” ibi byavuzwe na Priyanshi Rana, umwanditsi wa mbere akaba n'umunyeshuri warangije mu itsinda ry'ubushakashatsi rya Lahey. Dukurikije igitekerezo cyabo, abashakashatsi basanze kubuza DELLA bishobora kugarura cyane imiterere y'imikurire n'imikurire y'ibimera bya MpVIH bitameze neza. Ibi byavuye mu bushakashatsi bigaragaza ko VIH kinase igenga nabi DELLA, igateza imbere imikurire n'imikurire y'ibimera.
Ubushakashatsi kuri poroteyine za DELLA bwatangiye kuva mu gihe cy’impinduramatwara y’ibidukikije, aho abahanga mu bya siyansi bakoresheje ubushobozi bwabo batabizi kugira ngo bakore ubwoko bw’ibimera bitanga umusaruro mwinshi. Nubwo ibisobanuro birambuye ku byo bakoze bitari byumvikana neza icyo gihe, ikoranabuhanga rigezweho ryemerera abahanga mu bya siyansi guhindura imikorere y’izo poroteyine binyuze mu buhanga mu by’uturemangingo, bigatuma umusaruro w’ibihingwa wiyongera neza.