Abashakashatsi bavumbuye uburyo bwo kugenzura poroteyine ya DELLA mu bimera.

Abashakashatsi bo mu ishami ry’ibinyabuzima mu Ishuri Rikuru ry’Ubumenyi ry’Ubuhinde (IISc) bavumbuye uburyo bumaze igihe kirekire bukoreshwa n’ibimera by’ubutaka bwa mbere nka bryofitike (harimo na mose hamwe n’umwijima) kugezakugenga imikurire y'ibihingwa- uburyo bwanabitswe mu bimera byindabyo biherutse guhinduka.

Ubu bushakashatsi bwasohotse mu kinyamakuru Nature Chemical Biology, bwibanze ku mabwiriza adasanzwe ya poroteyine ya DELLA, igenzura rikomeye rishobora kubuza igabana ry'uturemangingo mu bimera (ibimera byo ku butaka).
Debabrata Laha, umwarimu wungirije w’ibinyabuzima akaba n’umwanditsi w’ubwo bushakashatsi abisobanura agira ati: “DELLA ikora nk'umuvuduko ukabije, ariko niba iyi umuvuduko uhoraho, igihingwa ntigishobora kugenda.” Kubwibyo, kwangirika kwa poroteyine za DELLA ni ingenzi mu kuzamura imikurire y’ibihingwa. Mu bimera byindabyo, DELLA yangirika iyo phytohormonegibberellin (GA)Ihuza na reseptor yayo GID1, ikora urwego GA-GID1-DELLA. Ibikurikira, proteine ​​ya DELLA repressor ihuza iminyururu ya ubiquitin kandi iteshwa agaciro na proteasome ya 26S.
Igishimishije, bryofite yari mubihingwa byambere byakolonije ubutaka, hashize imyaka miriyoni 500. Nubwo zitanga phytohormone gibberellin (GA), ibura reseptor ya GID1. Ibi bitera kwibaza: gukura niterambere ryibi bimera byubutaka byateganijwe gute?
Abashakashatsi bifashishije sisitemu ya CRISPR-Cas9 kugirango bakureho gene ihuye na VIH, bityo bemeza uruhare rwa VIH. Ibimera bidafite imisemburo ya VIH ikora bikura bikura cyane nudusembwa twiterambere ndetse na morphologie idasanzwe, nka thallus yuzuye, kubangamira imikurire ya radiyo, no kubura calyx. Izi nenge zarakosowe muguhindura genome yibihingwa kugirango itange impera imwe gusa (N-terminus) ya enzyme ya VIH. Hifashishijwe tekinoroji ya chromatografiya, itsinda ryubushakashatsi ryavumbuye ko N-terminus irimo domaine ya kinase itanga umusaruro wa InsP₈.
Abashakashatsi bavumbuye ko DELLA ari imwe mu ntego za selile ya VIH kinase. Byongeye kandi, babonye ko phenotype y’ibimera bidafite MpVIH bisa n’ibimera bya Miscanthus multiforme hamwe n’imvugo ya DELLA yiyongereye.
Priyanshi Rana, umunyeshuri wa dogiteri mu itsinda ry’ubushakashatsi bwa Lahey akaba n'umwanditsi wa mbere w'iki kinyamakuru yagize ati: "Kuri iki cyiciro, dushishikajwe no kumenya niba DELLA itajegajega cyangwa ibikorwa byongerewe imbaraga mu bimera bidafite MpVIH." Bihuye na hypothesis, abashakashatsi basanze kubuza DELLA byagaruye cyane imikurire niterambere ryibihingwa bya mutant MpVIH. Ubu bushakashatsi bwerekana ko VIH kinase igenga DELLA nabi, bityo igatera imbere gukura niterambere.
Abashakashatsi bahujije uburyo bwa genetike, ibinyabuzima, na biofiziki kugira ngo basobanure uburyo inositol pyrophosphate igenga imvugo ya poroteyine ya DELLA muri iyi bryophyte. By'umwihariko, InsP₈, yakozwe na MpVIH, ihuza poroteyine ya MpDELLA, iteza imbere polyubiquitination yayo, ari na yo iganisha ku kwangirika kwa poroteyine ya repressor na proteasome.
Ubushakashatsi kuri poroteyine ya DELLA bwatangiriye kuri Green Revolution, igihe abahanga batabizi bakoresheje ubushobozi bwayo bwo gukora ubwoko butanga umusaruro mwinshi wa dwarf. Nubwo icyo gihe imikorere yacyo itari izwi, ikoranabuhanga rigezweho ryatumye abahanga mu bya siyanse bakoresha genoside kugira ngo bakoreshe imikorere ya poroteyine, bityo umusaruro wibihingwa wiyongere.
Raha yagize ati: "Ubwiyongere bw'abaturage no kugabanuka k'ubutaka bwo guhinga, kongera umusaruro w'ibihingwa byabaye ingirakamaro". Urebye ko kwangirika kwa DELLA kugenzurwa na InsP₈ bishobora gukwirakwira mu bimera byo mu nsoro, ubu buvumbuzi bushobora guha inzira iterambere ry’ibisekuru bizaza bitanga umusaruro mwinshi.