Imikorere ya Uniconazole

       Uniconazoleni triazolekugenzura imikurire yikimeraibyo bikoreshwa cyane muguhuza uburebure bwibimera no gukumira ingemwe zikura. Nyamara, uburyo bwa molekile uburyo uniconazole ibuza gutera ingemwe ya hypocotyl ntikiramenyekana neza, kandi hariho ubushakashatsi buke buhuza amakuru ya transcriptome na metabolome kugirango hakorwe iperereza ku buryo bwo kurambura hypocotyl. Hano, twabonye ko uniconazole yabujije cyane kurambura hypocotyl mu ngemwe z’indabyo zo mu Bushinwa. Igishimishije, dushingiye ku isesengura rya transcriptome na metabolome, twasanze uniconazole yagize ingaruka zikomeye kumuhanda wa "fenylpropanoid biosynthesis". Muri iyi nzira, gene imwe gusa yumuryango wa enzyme igenga gene, BrPAL4, igira uruhare muri lignin biosynthesis, yagabanijwe cyane. Byongeye kandi, umusemburo umwe-hybrid hamwe na bibiri-bivangavanze byerekanaga ko BrbZIP39 ishobora guhita ihuza akarere ka porotokoro ya BrPAL4 kandi igakora transcript yayo. Sisitemu yo gucecekesha virusi iterwa na virusi yongeye kwerekana ko BrbZIP39 ishobora kugenzura neza kurambura hypocotyl kurambura imyumbati yo mu Bushinwa hamwe na hypocotyl lignin synthesis. Ibyavuye muri ubu bushakashatsi bitanga ubumenyi bushya muburyo bwo kugenzura imikorere ya cloconazole muguhagarika hypocotyl kurambura imyumbati yabashinwa. Ku nshuro ya mbere hemejwe ko cloconazole yagabanije lignine ibuza synthesis ya fenylpropanoide yahujwe na module ya BrbZIP39-BrPAL4, bityo bigatuma habaho hypocotyl dwarfing mu ngemwe z’imyumbati mu Bushinwa.

Imyumbati y'Abashinwa (Brassica campestris L. ssp. Chinensis var. Useis Tsen et Lee) ni iy'ubwoko bwa Brassica kandi ni imboga zizwi cyane buri mwaka zihingwa cyane mu gihugu cyanjye (Wang et al., 2022; Yue et al., 2022). Mu myaka yashize, igipimo cy’umusaruro w’indabyo zo mu Bushinwa cyakomeje kwaguka, kandi uburyo bwo guhinga bwahindutse buva mu mbuto gakondo butaziguye bugera ku muco w’ingemwe no guhingwa. Nyamara, mugihe cyumuco wimbuto wimbuto no guhindurwa, gukura cyane kwa hypocotyl bikunda gutanga ingemwe zumuti, bikavamo ubwiza bwingemwe. Kubwibyo rero, kugenzura imikurire ikabije ya hypocotyl nikibazo gikomeye mumico yo gutera ingemwe no guhinduranya imyumbati yabashinwa. Kugeza ubu, hari ubushakashatsi buke buhuza transcriptomics na metabolomics data kugirango tumenye uburyo bwo kurambura hypocotyl. Uburyo bwa molekile ikoreshwa na chlorantazole igenga kwaguka kwa hypocotyl mu myumbati y'Ubushinwa ntabwo byigeze byigwa. Twari tugamije kumenya genes n'inzira za molekuline zisubiza hypocotyl iterwa na uniconazole iterwa na cabage y'Ubushinwa. Twifashishije isesengura rya transcriptome na metabolomic, hamwe n'umusemburo umwe wa Hybrid, isesengura rya luciferase ebyiri, hamwe na virusi iterwa na virusi (VIGS), twasanze uniconazole ishobora gutera hypocotyl dwarfing mu myumbati y'Ubushinwa mu kubuza lignin biosynthesis mu ngemwe z'imyumbati y'Abashinwa. Ibisubizo byacu bitanga ubumenyi bushya muburyo bwo kugenzura imikorere ya molekuline uniconazole ibuza hypocotyl kuramba muri cabage yubushinwa binyuze mukubuza fenilpropanoid biosynthesis yahujwe na module ya BrbZIP39 - BrPAL4. Ibisubizo birashobora kugira ingaruka zifatika zo kuzamura ubwiza bwingemwe zubucuruzi no kugira uruhare mukumenya umusaruro nubwiza bwimboga.
Uburebure bwuzuye BrbZIP39 ORF yinjijwe muri pGreenll 62-SK kugirango itange effektor, hanyuma agace ka porotokoro ya BrPAL4 kahujwe na gene yumunyamakuru pGreenll 0800 luciferase (LUC) kugirango habeho gene yumunyamakuru. Imikorere n'umunyamakuru gene vectors yahinduwe mubibabi byitabi (Nicotiana benthamiana).
Kugirango dusobanure isano ya metabolite na genes, twakoze isesengura rya metabolome hamwe na transcriptome. Isesengura ryinzira ya KEGG ryerekanye ko DEGs na DAM byafatanyirijwe hamwe mumihanda 33 ya KEGG (Ishusho 5A). Muri byo, inzira ya "fenylpropanoid biosynthesis" niyo yari ikungahaye cyane; inzira ya "fotosintetike ya karubone ikosora", inzira ya "flavonoid biosynthesis", inzira ya "pentose-glucuronic acide interconversion", inzira ya "tryptophan metabolism", n'inzira ya "starch-sucrose metabolism" nayo yari ikungahaye cyane. Ikarita yo gukusanya ubushyuhe (Igicapo 5B) yerekanaga ko DAM ifitanye isano na DEGs yagabanyijwemo ibyiciro byinshi, muri byo flavonoide ikaba ari cyo cyiciro kinini, byerekana ko inzira ya “fenylpropanoid biosynthesis” yagize uruhare runini muri dwarfism ya hypocotyl.
Abanditsi batangaza ko ubushakashatsi bwakozwe mu gihe nta mibanire y’ubucuruzi cyangwa iy'imari ishobora kumvikana nk'amakimbirane ashobora guterwa n'inyungu.
Ibitekerezo byose byavuzwe muriyi ngingo ni iby'umwanditsi gusa kandi ntibigaragaza byanze bikunze ibitekerezo byimiryango ifitanye isano, abamamaza, abanditsi, cyangwa abasesengura. Ibicuruzwa byose byasuzumwe muriyi ngingo cyangwa ibyifuzo byatanzwe nababikoze ntabwo byemewe cyangwa byemejwe nuwamamaza.