Imikorere ya Uniconazole

       Uniconazoleni triazoleigenzura ry'imikurire y'ibimeraIbyo bikoreshwa cyane mu kugenzura uburebure bw'ibimera no gukumira gukura kw'ibimera. Ariko, uburyo bwa molekile uniconazole butuma ibimera bigabanya uburebure bwa hypocotyl ntabwo busobanutse neza, kandi hari ubushakashatsi bucye buhuza amakuru ya transcriptome na metabolome kugira ngo hamenyekane uburyo hypocotyl yagutse. Aha, twabonye ko uniconazole yabujije cyane uburebure bwa hypocotyl mu bimera by'amashu by'Abashinwa. Igishimishije ni uko hashingiwe ku isesengura rya transcriptome na metabolome, twasanze uniconazole yagize ingaruka zikomeye ku nzira ya "phenylpropanoid biosynthesis". Muri iyi nzira, gene imwe gusa yo mu muryango wa enzyme regulatory gene, BrPAL4, ifite uruhare mu gukora lignin biosynthesis, yagabanutse cyane. Byongeye kandi, ibizamini bya yeast one-hybrid na two-hybrid byagaragaje ko BrbZIP39 ishobora gufatana neza n'agace k'umuvuduko wa BrPAL4 no gukora transcription yayo. Uburyo bwo gucecekesha jeni buterwa na virusi bwagaragaje ko BrbZIP39 ishobora kugenzura neza ubwiyongere bwa hypocotyl bw'ikyunguruzo cy'ikyunguruzo cy'Abashinwa n'ubwiyongere bwa hypocotyl lignin. Ibisubizo by'ubu bushakashatsi bitanga ibisobanuro bishya ku buryo bwo kugenzura molekile ya cloconazole mu gukumira ubwiyongere bwa hypocotyl bw'ikyunguruzo cy'Abashinwa. Byemejwe bwa mbere ko cloconazole yagabanije ingano ya lignin ibuza ubwiyongere bwa phenylpropanoid buterwa na module ya BrbZIP39-BrPAL4, bityo bigatera ubwiyongere bwa hypocotyl mu mbuto z'ikyunguruzo z'Abashinwa.

Ishu y'Abashinwa (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) iri mu bwoko bwa Brassica kandi ni imboga zizwi cyane zihingwa buri mwaka mu gihugu cyanjye (Wang et al., 2022; Yue et al., 2022). Mu myaka ya vuba aha, umusaruro wa cauliflower yo mu Bushinwa wakomeje kwiyongera, kandi uburyo bwo guhinga bwarahindutse kuva ku gutera imbuto mu buryo busanzwe bujya mu gutera imbuto nyinshi no gutera. Ariko, mu gihe cyo gutera imbuto nyinshi no gutera imbuto nyinshi, gukura cyane kwa hypocotyl bikunda gutanga imbuto zikomeye, bigatuma imbuto zimera nabi. Kubwibyo, kugenzura gukura cyane kwa hypocotyl ni ikibazo gikomeye mu gutera imbuto nyinshi no gutera ishu y'Abashinwa. Kuri ubu, hari ubushakashatsi buke buhuriweho mu gusesengura uburyo bwo kurekura kwa hypocotyl. Uburyo bwa molekile chlorantazole ikoresha mu kugenzura kwaguka kwa hypocotyl mu ishu y'Abashinwa ntiburakorwa. Twari tugamije kumenya imiterere y’uturemangingo n’inzira za molekile zisubiza hypocotyl dwarfing iterwa na uniconazole mu ikayi y’Abashinwa. Dukoresheje isesengura rya transcriptome na metabolomic, hamwe n’isesengura rya yeast one-hybrid, isuzuma rya dual luciferase, n’isuzuma rya gene silencing iterwa na virusi (VIGS), twasanze uniconazole ishobora gutera hypocotyl dwarfing mu ikayi y’Abashinwa binyuze mu kubuza lignin biosynthesis mu mbuto z’ikayi y’Abashinwa. Ibisubizo byacu bitanga ubumenyi bushya ku buryo bwo kugenzura molekile uniconazole ibuza hypocotyl elongation mu ikayi y’Abashinwa binyuze mu kubuza phenylpropanoid biosynthesis iterwa na module ya BrbZIP39–BrPAL4. Ibi bisubizo bishobora kugira ingaruka zikomeye mu kunoza ubwiza bw’imbuto z’ubucuruzi no gutanga umusanzu mu kwemeza umusaruro n’ubwiza bw’imboga.
BrbZIP39 ORF yuzuye yashyizwe muri pGreenll 62-SK kugira ngo hakorwe effector, maze igice cya promoter cya BrPAL4 gishyirwa kuri pGreenll 0800 luciferase (LUC) reporter gene kugira ngo hakorwe reporter gene. Effector na reporter gene vectors byahinduwe hamwe mu mababi y'itabi (Nicotiana benthamiana).
Kugira ngo dusobanure isano iri hagati ya metabolites na genes, twakoze isesengura rya metabolome na transcriptome. Isesengura rya KEGG ryongereye inzira ryagaragaje ko DEG na DAM byakungahaye ku buryo bungana mu nzira 33 za KEGG (Ishusho ya 5A). Muri zo, inzira ya "phenylpropanoid biosynthesis" niyo yakungahaye cyane; inzira ya "fositike ya karuboni", inzira ya "flavonoid biosynthesis", inzira ya "pentose-glucuronic acid interconversion", inzira ya "tryptophan metabolism", n'inzira ya "starch-sucrose metabolism" nabyo byakungahaye cyane. Ikarita y'ubushyuhe (Ishusho ya 5B) yagaragaje ko DAM zifitanye isano na DEG zagabanijwemo ibyiciro byinshi, muri byo flavonoids zari icyiciro kinini, bigaragaza ko inzira ya "phenylpropanoid biosynthesis" yagize uruhare runini mu kuba dwarfism ya hypocotyl.
Abanditsi batangaza ko ubushakashatsi bwakozwe mu gihe nta mibanire y’ubucuruzi cyangwa imari yari ihari ishobora gufatwa nk’igongana ry’inyungu.
Ibitekerezo byose bivugwa muri iyi nkuru ni iby’umwanditsi gusa kandi ntibigaragaza ibitekerezo by’imiryango ifitanye isano, abasohora ibitabo, abanditsi, cyangwa abasesengura. Ibicuruzwa byose byasuzumwe muri iyi nkuru cyangwa ibyavuzwe n’ababikora ntabwo byemejwe cyangwa ngo byemezwe n’uwabisohoye.