Igenzura ryimikurire 5-aminolevulinic aside yongera ubukonje bwibiti byinyanya.

      Nka kimwe mu bintu nyamukuru bihangayikisha abiotic, guhangayikishwa n'ubushyuhe buke bidindiza cyane imikurire y'ibihingwa kandi bigira ingaruka mbi ku musaruro n'ubwiza bw'ibihingwa.5-Acide Aminolevulinic (ALA) nigenzura ryimikurire igaragara cyane mubikoko n'ibimera.Bitewe nubushobozi buhanitse, kutagira uburozi no kwangirika byoroshye, bikoreshwa cyane mugikorwa cyo kwihanganira ubukonje bwibimera.
Nyamara, ubushakashatsi bwinshi bugezweho bujyanye na ALA bwibanda cyane cyane kugenzura imiyoboro yanyuma.Uburyo bwihariye bwa molekuline yibikorwa bya ALA mugihe cyo kwihanganira ubukonje hakiri kare ibimera ntibisobanutse kandi bisaba ubushakashatsi bwakozwe nabahanga.
Muri Mutarama 2024, Ubushakashatsi bw’imboga bwasohoye inyandiko y’ubushakashatsi yise “Acide 5-Aminolevulinic Yongera Ubworoherane Bw’Ubukonje mu kugena ubwoko bwa SlMYB4 / SlMYB88-SlGSTU43 Ubwoko bwa Oxygene Yangiza Moderi mu nyanya” n’itsinda rya Hu Xiaohui mu buhinzi n’amashyamba ya kaminuza ya Northwestern.
Muri ubu bushakashatsi, glutathione S-transfera gene SlGSTU43 yagaragaye mu nyanya (Solanum lycopersicum L.).Ibyavuye mu bushakashatsi byagaragaje ko ALA itera cyane imvugo ya SlGSTU43 munsi yubukonje bukabije.Imirongo y'inyanya ya transgenque ikabije SlGSTU43 yerekanye imbaraga ziyongera cyane mubwoko bwa ogisijeni ikora kandi igaragaza ko irwanya cyane ubushyuhe buke, mugihe imirongo ya mutant ya SlGSTU43 yumvaga ubushyuhe buke.
Byongeye kandi, ibyavuye mu bushakashatsi byagaragaje ko ALA itongera kwihanganira imiterere ya mutant ku bushyuhe buke.Rero, ubushakashatsi bwerekana ko SlGSTU43 ari gene yingenzi mugikorwa cyo kongera kwihanganira ubukonje mu nyanya na ALA (Ishusho 1).
Byongeye kandi, ubu bushakashatsi bwemeje binyuze muri EMSA, Y1H, LUC na ChIP-qPCR ko SlMYB4 na SlMYB88 zishobora kugenzura imvugo ya SlGSTU43 muguhuza na porotokoro ya SlGSTU43.Ubundi bushakashatsi bwerekanye ko SlMYB4 na SlMYB88 nabo bagize uruhare muri gahunda ya ALC mu kongera kwihanganira inyanya guhangana n’ubushyuhe buke no kugenzura neza imvugo ya SlGSTU43 (Ishusho 2).Ibisubizo biratanga ubumenyi bushya muburyo ALA yongerera kwihanganira ubushyuhe buke mubinyanya.
Andi makuru: Zhengda Zhang nabandi, aside-aminolevulinic 5 yongerera kwihanganira ubukonje muguhindura module ya SlMYB4 / SlMYB88-SlGSTU43 kubwoko bwa ogisijeni ikora neza inyanya mu nyanya, Ubushakashatsi bwimbuto (2024).DOI: 10.1093 / isaha / uhae026
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