Imihindagurikire y’ibihe n’ubwiyongere bw’abaturage byabaye imbogamizi ku kwihaza mu biribwa ku isi.Igisubizo kimwe gitanga ikizere ni ugukoreshaibimera bikura(PGRs) kongera umusaruro wibihingwa no gutsinda ibihe bibi byo gukura nkibihe byubutayu.Vuba aha, karotenoid zaxinone hamwe na bibiri bisa nayo (MiZax3 na MiZax5) byagaragaje ibikorwa biteza imbere iterambere mu bihingwa by’ibinyampeke n’imboga mu gihe cya pariki n’imirima.Hano, twakomeje gukora ubushakashatsi ku ngaruka ziterwa na MiZax3 na MiZax5 (5 μM na 10 μM muri 2021; strawberry.Arabiya.Mu bigeragezo bitanu byigenga byakozwe kuva 2021 kugeza 2022, ikoreshwa rya MiZax ryateje imbere cyane ibihingwa ngandurarugo, ibice byumusaruro numusaruro rusange.Birakwiye ko tumenya ko MiZax ikoreshwa mukigero cyo hasi cyane kuruta aside humic (uruganda rukoreshwa cyane mubucuruzi rukoreshwa hano kugereranya).Rero, ibisubizo byacu byerekana ko MiZax ari igenzura ryiza ryikura ryibihingwa rishobora gukoreshwa mugutezimbere imikurire numusaruro wibihingwa byimboga ndetse no mubutayu ndetse no mubutumburuke buke.
Ishami ry'Umuryango w'Abibumbye ryita ku biribwa n'ubuhinzi (FAO) rivuga ko gahunda yo gutanga ibiribwa igomba kuba hafi gatatu mu 2050 kugira ngo igaburire abatuye isi biyongera (FAO: Isi izakenera ibiryo 70% mu 20501).Mubyukuri, ubwiyongere bwabaturage bwihuse, umwanda, ibyonnyi by’udukoko cyane cyane ubushyuhe bwinshi n’amapfa biterwa n’imihindagurikire y’ikirere ni ibibazo byose byugarije umutekano w’ibiribwa ku isi2.Ni muri urwo rwego, kongera umusaruro mwinshi w’ibihingwa by’ubuhinzi mu bihe bidasanzwe ni kimwe mu bisubizo bidashidikanywaho kuri iki kibazo cy’ingutu.Nyamara, gukura kw'ibimera no gutera imbere biterwa ahanini no kubona intungamubiri mu butaka kandi bikabuzwa cyane n’ibidukikije byangiza ibidukikije, birimo amapfa, umunyu cyangwa guhangayikishwa n’ibinyabuzima3,4,5.Izi mpungenge zirashobora kugira ingaruka mbi kubuzima niterambere ryibihingwa hanyuma amaherezo bigatuma umusaruro wibihingwa ugabanuka6.Byongeye kandi, umutungo w’amazi meza ugira ingaruka zikomeye ku kuhira imyaka, mu gihe imihindagurikire y’ikirere ku isi byanze bikunze igabanya ubuso bw’ubutaka bwo guhingwa kandi ibintu nk’imivumba y’ubushyuhe bigabanya umusaruro w’ibihingwa7,8.Ubushyuhe bwo hejuru burasanzwe mu bice byinshi byisi, harimo na Arabiya Sawudite.Gukoresha ibinyabuzima cyangwa ibimera bikura (PGRs) ni ingirakamaro mu kugabanya ukwezi gukura no kongera umusaruro.Irashobora guteza imbere kwihanganira ibihingwa no gutuma ibimera bihanganira ibihe bibi bikura9.Ni muri urwo rwego, ibinyabuzima bigenga imikurire n’ibimera bishobora gukoreshwa mu buryo bwiza bwo kuzamura ibihingwa n’umusaruro10,11.
Carotenoide ni tetraterpenoide nayo ibanziriza aside ya phytohormone abscisic aside (ABA) na strigolactone (SL) 12,13,14, hamwe nabashinzwe kugenzura imikurire ya zaxinone, anorene na cyclocitral15,16,17,18,19.Nyamara, ibyinshi mubyukuri metabolite, harimo ibikomoka kuri karotenoide, bifite amasoko make kandi / cyangwa bidahindagurika, bigatuma kubishyira mubikorwa bitoroshye.Rero, mumyaka mike ishize, ibigereranyo byinshi bya ABA na SL bigereranijwe kandi bigeragezwa kubikorwa byubuhinzi20,21,22,23,24,25.Mu buryo nk'ubwo, duherutse gukora kwigana zaxinone (MiZax), metabolite itera imbere gukura ishobora kugira ingaruka mu kongera isukari metabolisme no kugenzura SL homeostasis mu mizi y'umuceri 19.26.Kwigana zaxinone 3 (MiZax3) na MiZax5 (imiterere yimiti yerekanwe ku gishushanyo 1A) yerekanaga ibikorwa byibinyabuzima bigereranywa na zaxinone mu bimera byumuceri wo mu gasozi bihingwa mu buryo bwa hydroponique no mu butaka26.Byongeye kandi, kuvura inyanya, imikindo, urusenda rwatsi nigihaza hamwe na zaxinone, MiZax3 na MiZx5 byateje imbere imikurire y’umusaruro n’umusaruro, ni ukuvuga umusaruro w’urusenda n’ubuziranenge, muri pariki n’ahantu hafunguye, byerekana uruhare rwabo nka biostimulants no gukoresha PGR27..Igishimishije, MiZax3 na MiZax5 nazo zateje imbere kwihanganira umunyu urusenda rwatsi rwahinzwe mugihe cyumunyu mwinshi, kandi MiZax3 yongereye zinc yimbuto zimbuto mugihe zashyizwe hamwe na zinc zirimo ibyuma-ngenga 7,28.
(A) Imiterere yimiti ya MiZax3 na MiZax5..Ubushakashatsi buzaba muri 2021. Amakuru yatanzwe asobanura ± SD.n≥15.Isesengura mibare ryakozwe hifashishijwe isesengura ryuburyo bumwe (ANOVA) hamwe na test ya Huke ya Tukey.Inyenyeri zerekana itandukaniro rinini cyane ugereranije no kwigana (* p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001; ns, ntabwo ari ngombwa).HA - aside humic;MZ3, MiZax3, MiZax5;HA - aside humic;MZ3, MiZax3, MiZax5;
Muri iki gikorwa, twasuzumye MiZax (MiZax3 na MiZax5) ku bibumbe bitatu by'amababi (5 µM na 10 µM muri 2021 na 2.5 µM na 5 µM muri 2022) tubigereranya n'ibirayi (Solanum tuberosum L).Acide igabanya ubukana bwa acide humic (HA) yagereranijwe na strawberry (Fragaria ananassa) mu bigeragezo bya parike ya strawberry mu 2021 na 2022 no mu bigeragezo bine byakozwe mu bwami bwa Arabiya Sawudite, akarere gasanzwe k’ikirere.Nubwo HA ikoreshwa cyane na biostimulant hamwe ningaruka nyinshi zingirakamaro, harimo kongera intungamubiri ziboneka mubutaka no guteza imbere imikurire muguhindura imisemburo ya homeostasis, ibisubizo byacu byerekana ko MiZax iruta HA.
Ibijumba byubwoko bwa Diamond byaguzwe muri Jabbar Nasser Al Bishi Trading Company, Jeddah, Arabiya Sawudite.Imbuto z'ubwoko bubiri bwa strawberry “Sweet Charlie” na “Festival” na acide humic byaguzwe muri sosiyete igezweho ya Agritech, Riyadh, Arabiya Sawudite.Ibikoresho byose by’ibimera bikoreshwa muri iki gikorwa byujuje ibyavuzwe na Politiki ya IUCN ku bushakashatsi bujyanye n’ibinyabuzima bigenda byangirika ndetse n’amasezerano y’ubucuruzi mu bwoko bw’inyamanswa zo mu gasozi n’ibimera.
Ikibanza cyubushakashatsi giherereye muri Hada Al-Sham, Arabiya Sawudite (21 ° 48′3 ″ N, 39 ° 43′25 ″ E).Ubutaka ni umusenyi, pH 7.8, EC 1.79 dcm-130.Imiterere yubutaka irerekanwa mumeza yinyongera S1.
Ingemwe eshatu (Fragaria x ananassa D. var. Ibirori) ingemwe kurwego rwibibabi nyabyo zagabanijwemo amatsinda atatu kugirango harebwe ingaruka ziterwa no gutera amababi hamwe na 10 μM MiZax3 na MiZax5 kubiranga imikurire nigihe cyo kurabyo mubihe bya parike.Gutera amababi n'amazi (arimo 0.1% acetone) yakoreshejwe muburyo bwo kwerekana icyitegererezo.MiZax foliar spray yakoreshejwe inshuro 7 mugihe cyicyumweru.Ubushakashatsi bubiri bwigenga bwakozwe ku ya 15 na 28 Nzeri 2021.Igipimo cyo gutangira cya buri ruganda ni ml 50 hanyuma kigenda cyiyongera buhoro buhoro kugeza kuri 250 ya nyuma.Mugihe cibyumweru bibiri bikurikiranye, umubare wibimera byindabyo byandikwa buri munsi kandi igipimo cy’indabyo cyabazwe mu ntangiriro zicyumweru cya kane.Kugirango umenye ibiranga imikurire, umubare wibabi, gutera ibimera bishya kandi byumye, ubuso bwamababi yose, hamwe numubare wintebe kuri buri gihingwa byapimwe nyuma yicyiciro cyo gukura no gutangira icyiciro cyimyororokere.Agace k'amababi gapimwe hifashishijwe metero yikibabi hanyuma ingero nshya zumishwa mu ziko kuri 100 ° C mumasaha 48.
Ibigeragezo bibiri byo mu murima byakozwe: guhinga kare na nyuma.Ibijumba byubwoko bwa "Diamant" byatewe mu Gushyingo na Gashyantare, hamwe nibihe byera kandi bitinze.Biostimulants (MiZax-3 na -5) ikoreshwa muburyo bwa 5.0 na 10.0 µM (2021) na 2.5 na 5.0 µM (2022).Koresha aside humic (HA) 1 g / l inshuro 8 mu cyumweru.Amazi cyangwa acetone yakoreshejwe nkigenzura ribi.Igishushanyo mbonera cyibizamini byerekanwe muri (Ishusho yinyongera S1).Igishushanyo mbonera cyuzuye cyo guhagarika (RCBD) gifite ubuso bwa metero 2,5 m × 3.0 m cyakoreshejwe mugukora ubushakashatsi.Buri miti yasubiwemo inshuro eshatu nkuko yigenga yigana.Intera iri hagati ya buri kibanza ni 1.0 m, naho intera iri hagati ya buri gice ni 2.0 m.Intera iri hagati yibimera ni 0,6 m, intera iri hagati yumurongo ni m 1.Ibihingwa byibirayi byuhira buri munsi bitonyanga ku gipimo cya 3,4 l kuri buri gitonyanga.Sisitemu ikora kabiri kumunsi muminota 10 buri mwanya kugirango itange amazi kubihingwa.Uburyo bwose busabwa ubuhinzi bwubuhinzi bwo guhinga ibirayi mugihe cyamapfa bwakoreshejwe31.Amezi ane nyuma yo gutera, uburebure bwibihingwa (cm), umubare wamashami kuri buri gihingwa, ibijumba numusaruro, hamwe nubuziranenge bwibijumba byapimwe hakoreshejwe tekiniki zisanzwe.
Imbuto zubwoko bubiri bwa strawberry (Sweet Charlie na Festival) zapimwe mubihe byumurima.Biostimulants (MiZax-3 na -5) yakoreshejwe nk'ibibabi bitera amababi ya 5.0 na 10.0 µM (2021) na 2.5 na 5.0 µM (2022) inshuro umunani mu cyumweru.Koresha 1 g ya HA kuri litiro nka spray yamababi ugereranije na MiZax-3 na -5, hamwe na H2O igenzura ivanze cyangwa acetone nkigenzura ribi.Ingemwe za strawberry zatewe mu kibanza cya 2,5 x 3 m mu ntangiriro zUgushyingo gifite igihingwa gifite metero 0,6 na intera ya metero 1.Ubushakashatsi bwakorewe muri RCBD kandi bwasubiwemo inshuro eshatu.Ibimera byuhira iminota 10 buri munsi saa moya na 17h00 hakoreshejwe uburyo bwo kuhira ibitonyanga birimo ibitonyanga bifite metero 0,6 hagati kandi bifite ubushobozi bwa 3.4 L. Ibikoresho by’ubuhinzi n’ibipimo by’umusaruro byapimwe mu gihe cy’ihinga.Ubwiza bwimbuto burimo TSS (%), vitamine C32, acide hamwe nibintu byose bya fenolike33 byasuzumwe muri Laboratoire ya Physiologiya n’ikoranabuhanga rya Postharvest Physiology na Technology ya kaminuza ya King Abdulaziz.
Ibyatanzwe bigaragazwa nkuburyo kandi gutandukana bigaragazwa nkibisanzwe.Ubusobanuro bwibarurishamibare bwagenwe hakoreshejwe inzira imwe ya ANOVA (inzira imwe ANOVA) cyangwa ANOVA yinzira ebyiri ukoresheje ikizamini cyo kugereranya inshuro nyinshi ya Tukey ukoresheje urwego rushoboka rwa p <0.05 cyangwa ikizamini cya t umurizo wibizamini bibiri kugirango umenye itandukaniro rikomeye (* p <0.05 , * * p <0.01, *** p <0.001, **** p <0.0001).Ibisobanuro byose bibarurishamibare byakozwe hakoreshejwe GraphPad Prism verisiyo 8.3.0.Amashyirahamwe yageragejwe hifashishijwe isesengura ryibanze (PCA), uburyo butandukanye bwibarurishamibare, ukoresheje R pack 34.
Muri raporo yabanjirije iyi, twerekanye ibikorwa biteza imbere iterambere rya MiZax kuri 5 na 10 μM yibanda ku bimera by’indabyo kandi tunonosora icyerekezo cya chlorophyll mu butaka bw’ubutaka (SPAD) 27.Dufatiye kuri ibyo bisubizo, twakoresheje ibitekerezo bimwe kugira ngo dusuzume ingaruka za MiZax ku birayi, igihingwa cy’ibiribwa ku isi, mu bigeragezo byo mu kirere mu bihe by’ubutayu mu 2021. By'umwihariko, twashishikajwe no gusuzuma niba MiZax ishobora kongera kwirundanya kwa krahisi , ibicuruzwa byanyuma bya fotosintezeza.Muri rusange, ikoreshwa rya MiZax ryateje imbere imikurire y ibihingwa byibirayi ugereranije na aside humic (HA), bituma ubwiyongere bwibimera, biomass numubare wamashami (Ishusho 1B).Twongeyeho, twabonye ko 5 μM MiZax3 na MiZax5 byagize ingaruka zikomeye mu kongera uburebure bw’ibimera, umubare w’amashami, na biyomasi y’ibimera ugereranije na 10 μM (Ishusho 1B).Hamwe no gukura neza, MiZax nayo yongereye umusaruro, ugereranije numubare nuburemere bwibijumba byasaruwe.Ingaruka zingirakamaro muri rusange ntizagaragaye cyane mugihe MiZax yatangwaga ku gipimo cya 10 μM, byerekana ko ibyo bikoresho bigomba gutangwa ku bitekerezo biri munsi yibi (Ishusho 1B).Twongeyeho, twabonye ko nta tandukaniro riri mu bipimo byose byanditswe hagati y’ubuvuzi bwa acetone (urw'agashinyaguro) n’amazi (kugenzura), byerekana ko ingaruka zo guhindura imikurire zagaragaye zitatewe n’umuti, ibyo bikaba bihuye na raporo yacu yabanjirije27.
Kubera ko igihe cyo guhinga ibirayi muri Arabiya Sawudite kigizwe no gukura hakiri kare kandi bitinze, twakoze ubushakashatsi bwa kabiri mu murima mu 2022 dukoresheje imbaraga nke (2.5 na 5 µM) mu bihe bibiri kugirango dusuzume ingaruka zigihe cyimirima ifunguye (Ishusho yinyongera S2A).Nkuko byari byitezwe, porogaramu zombi za 5 μM MiZax zatanze umusaruro utera imbere gukura nkikigeragezo cya mbere: kongera uburebure bwibimera, kongera amashami, biomass nyinshi, no kongera umubare wibijumba (Ishusho 2; Ishusho yinyongera. S3).Icy'ingenzi, twabonye ingaruka zikomeye ziyi PGR ku gipimo cya 2.5 μM, mu gihe ubuvuzi bwa GA buterekanye ingaruka zahanuwe.Igisubizo cyerekana ko MiZax ishobora gukoreshwa no mubitekerezo bito kuruta uko byari byitezwe.Mubyongeyeho, porogaramu ya MiZax nayo yongereye uburebure nubugari bwibijumba (Ishusho yinyongera S2B).Twabonye kandi ubwiyongere bukabije bwibiro byibijumba, ariko 2,5 µM yibanze byakoreshejwe gusa mubihe byombi byo gutera;
Isuzuma rya fenotipiki yerekana ingaruka za MiZax ku bihingwa byibirayi bikuze hakiri kare mu murima wa KAU, byakozwe mu 2022. Amakuru yerekana ± gutandukana bisanzwe.n≥15.Isesengura mibare ryakozwe hifashishijwe isesengura ryuburyo bumwe (ANOVA) hamwe na test ya Huke ya Tukey.Inyenyeri zerekana itandukaniro rinini cyane ugereranije no kwigana (* p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001; ns, ntabwo ari ngombwa).HA - aside humic;MZ3, MiZax3, MiZax5;HA - aside humic;MZ3, MiZax3, MiZax5;
Kugirango usobanukirwe neza ingaruka zokuvurwa (T) numwaka (Y), inzira ebyiri ANOVA yakoreshejwe mugusuzuma imikoranire yabo (T x Y).Nubwo ibinyabuzima byose (T) byongereye cyane uburebure bwibihingwa byibirayi na biomass, gusa MiZax3 na MiZax5 byongereye cyane umubare wibijumba nuburemere, byerekana ko ibisubizo byombi byibirayi byibirayi kuri MiZax byombi byari bisa cyane (Ishusho 3)).Byongeye kandi, mu ntangiriro yigihembwe ikirere (https://www.timeanddate.com/weather/saudi-arabia/jeddah/climate) kirashyuha (ikigereranyo cya 28 ° C nubushuhe 52% (2022), bigabanya cyane muri rusange biomass biomass (Igishusho 2; Ishusho yinyongera. S3).
Wige ingaruka zo kuvura 5 µm (T), umwaka (Y) n'imikoranire yabo (T x Y) kubirayi.Amakuru yerekana ± gutandukana bisanzwe.n ≥ 30. Isesengura mibare ryakozwe hifashishijwe isesengura ryibice bibiri bitandukanye (ANOVA).Inyenyeri zerekana itandukaniro rinini cyane ugereranije no kwigana (* p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001; ns, ntabwo ari ngombwa).HA - aside humic;MZ3, MiZax3, MiZax5;
Nyamara, kuvura Myzax biracyakunda gutera imikurire y'ibihingwa bitinze.Muri rusange, ubushakashatsi bwacu butatu bwigenga bwerekanye nta gushidikanya ko ikoreshwa rya MiZax rifite ingaruka zikomeye ku miterere y’ibimera byongera umubare w’amashami.Mubyukuri, hari ingaruka zingenzi zuburyo bubiri hagati ya (T) na (Y) kumubare wamashami nyuma yo kuvura MiZax (Ishusho 3).Igisubizo kirahuye nibikorwa byabo nkibintu bibi bigenga strigolactone (SL) biosynthesis26.Byongeye kandi, twerekanye mbere ko kuvura Zaxinone bitera kwirundanya kwa krahisi mu mizi yumuceri35, bishobora gusobanura ubwiyongere bwubunini nuburemere bwibijumba nyuma yubuvuzi bwa MiZax, kubera ko ibirayi ahanini bigizwe na krahisi.
Ibihingwa byimbuto nibihingwa byingenzi byubukungu.Strawberry yunvikana kumiterere ya abiotic nkamapfa nubushyuhe bwinshi.Kubwibyo, twasesenguye ingaruka za MiZax kuri strawberry dutera amababi.Twabanje gutanga MiZax kumurongo wa 10 µM kugirango dusuzume ingaruka zayo kumikurire ya strawberry (Umunsi mukuru wimbuto).Igishimishije, twabonye ko MiZax3 yongereye cyane umubare wamabuye, ahwanye n’ishami ryiyongera, mu gihe MiZax5 yazamuye igipimo cy’indabyo, ibimera by’ibimera, hamwe n’ahantu h’ibabi mu bihe bya pariki (Ishusho yinyongera S4), byerekana ko ibyo bice byombi bishobora gutandukana mubinyabuzima.Ibyabaye 26.27.Kugira ngo turusheho gusobanukirwa n'ingaruka zabyo kuri strawberry mubuzima busanzwe bwubuhinzi, twakoze ibizamini byo mu murima dukoresha 5 na 10 μM MiZax ku bimera bya strawberry (cv. Sweet Charlie) bihingwa mu butaka bwumucanga mu 2021 (igicap. S5A).Ugereranije na GC, ntitwabonye ubwiyongere bwa biyomasi y'ibimera, ariko twabonye icyerekezo cyo kwiyongera k'imbuto (Ishusho C6A-B).Nyamara, gukoresha MiZax byatumye habaho kwiyongera cyane muburemere bwimbuto imwe kandi byerekana ko biterwa nubushakashatsi (Ishusho yinyongera S5B; Ishusho yinyongera S6B), byerekana ingaruka zibi bigenga imikurire yikimera kumiterere yimbuto za strawberry mugihe zikoreshwa mugihe cyubutayu.Ingaruka.
Kugira ngo twumve niba ingaruka zo kuzamura iterambere ziterwa n'ubwoko bw'ibihingwa, twahisemo ubwoko bubiri bw'ubucuruzi bwa strawberry muri Arabiya Sawudite (Sweet Charlie na Festival) kandi twakoze ubushakashatsi bubiri mu murima mu 2022 dukoresheje imbaraga nke za MiZax (2.5 na 5 µM).Kuri Sweet Charlie, nubwo umubare wimbuto zose utigeze wiyongera cyane, biomass yimbuto muri rusange yari myinshi kubihingwa bivurwa na MiZax, kandi umubare wimbuto kuri buri kibanza wiyongereye nyuma yo kuvura MiZax3 (Ishusho 4).Aya makuru yerekana kandi ko ibikorwa byibinyabuzima bya MiZax3 na MiZax5 bishobora gutandukana.Byongeye kandi, nyuma yo kuvurwa na Myzax, twabonye ubwiyongere bwuburemere bushya kandi bwumye bwibimera, hamwe nuburebure bwibihingwa.Kubyerekeranye numubare wibiti nibimera bishya, twasanze kwiyongera kuri 5 μM MiZax gusa (Ishusho 4), byerekana ko guhuza neza MiZax biterwa nubwoko bwibimera.
Ingaruka za MiZax ku miterere y'ibimera n'umusaruro wa strawberry (Ubwoko bwiza bwa Charlie) biva mu murima wa KAU, byakozwe mu 2022. Amakuru yerekana ± gutandukana bisanzwe.n ≥ 15, ariko umubare wimbuto kuri buri kibanza wabazwe ugereranije kuva ku bimera 15 biva mubibanza bitatu (n = 3).Isesengura mibare ryakozwe hifashishijwe isesengura ryuburyo bumwe (ANOVA) hamwe na Tukey's post hoc test cyangwa ikizamini cyumurizo ibiri.Inyenyeri zerekana itandukaniro rinini cyane ugereranije no kwigana (* p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001; ns, ntabwo ari ngombwa).HA - aside humic;MZ3, MiZax3, MiZax5;
Twabonye kandi ibikorwa bisa bikura bikura mubijyanye nuburemere bwimbuto hamwe na biyomasi yibihingwa muri strawberry yubwoko butandukanye bwibirori (Ishusho 5), ariko, ntitwabonye itandukaniro rigaragara mumibare yimbuto kuri buri gihingwa cyangwa kuri buri kibanza (Ishusho. 5);.Igishimishije, gukoresha MiZax byongereye uburebure bwibihingwa n’umubare w’ameza, byerekana ko ibyo bigenga imikurire y’ibimera bishobora gukoreshwa mu kuzamura imikurire y’imbuto (Ishusho 5).Twongeyeho, twapimye ibipimo byinshi bya biohimiki kugirango twumve ubwiza bwimbuto bwimbuto ebyiri zegeranijwe mu murima, ariko ntitwabonye itandukaniro riri hagati yubuvuzi bwose (Ishusho yinyongera S7; Ishusho yinyongera S8).
Ingaruka za MiZax kumiterere yibimera n'umusaruro wa strawberry mumurima wa KAU (Ibirori bitandukanye), 2022. Amakuru arasobanura iation gutandukana bisanzwe.n ≥ 15, ariko umubare wimbuto kuri buri kibanza wabazwe ugereranije kuva ku bimera 15 biva mubibanza bitatu (n = 3).Isesengura mibare ryakozwe hifashishijwe isesengura ryuburyo bumwe (ANOVA) hamwe na Tukey's post hoc test cyangwa ikizamini cyumurizo ibiri.Inyenyeri zerekana itandukaniro rinini cyane ugereranije no kwigana (* p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001; ns, ntabwo ari ngombwa).HA - aside humic;MZ3, MiZax3, MiZax5;
Mu bushakashatsi bwacu kuri strawberry, ibikorwa byibinyabuzima bya MiZax3 na MiZax5 byagaragaye ko bitandukanye.Twabanje gusuzuma ingaruka zo kuvura (T) numwaka (Y) kumurima umwe (Sweet Charlie) dukoresheje ANOVA yuburyo bubiri kugirango tumenye imikoranire yabo (T x Y).Niyo mpamvu, GA nta ngaruka yagize ku gihingwa cya strawberry (Sweet Charlie), mu gihe 5 μM MiZax3 na MiZax5 byiyongereye ku buryo bugaragara ibimera n'imbuto biomass (Ishusho 6), byerekana ko imikoranire y'inzira ebyiri za MiZax zombi zisa cyane mu kuzamura strawberry .umusaruro w'ibihingwa
Suzuma ingaruka zo kuvura 5 µM (T), umwaka (Y) n'imikoranire yabo (T x Y) kuri strawberry (cv. Sweet Charlie).Amakuru yerekana ± gutandukana bisanzwe.n ≥ 30. Isesengura mibare ryakozwe hifashishijwe isesengura ryibice bibiri bitandukanye (ANOVA).Inyenyeri zerekana itandukaniro rinini cyane ugereranije no kwigana (* p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001; ns, ntabwo ari ngombwa).HA - aside humic;MZ3, MiZax3, MiZax5;
Byongeye kandi, urebye ko ibikorwa bya MiZax kumoko yombi yari atandukanye gato (Ishusho 4; Igishusho 5), twakoze ANOVA yuburyo bubiri ugereranije no kuvura (T) nubwoko bubiri (C).Ubwa mbere, nta buvuzi bwagize ingaruka ku mubare w'imbuto kuri buri kibanza (Igicapo 7), byerekana ko nta mikoranire ihambaye iri hagati ya (T x C) kandi byerekana ko yaba MiZax cyangwa HA batagira uruhare mu mubare w'imbuto zose.Ibinyuranye, MiZax (ariko ntabwo HA) yongereye cyane uburemere bwibimera, uburemere bwimbuto, ameza hamwe n’ibiti bishya (Ishusho 7), byerekana ko MiZax3 na MiZax5 biteza imbere cyane imikurire y’ibihingwa bitandukanye bya strawberry.Dushingiye ku nzira ebyiri ANOVA (T x Y) na (T x C), dushobora kwemeza ko ibikorwa biteza imbere iterambere rya MiZax3 na MiZax5 mubihe byumurima bisa cyane kandi birahuye.
Isuzuma ryo kuvura strawberry hamwe na 5 µM (T), ubwoko bubiri (C) n'imikoranire yabyo (T x C).Amakuru yerekana ± gutandukana bisanzwe.n ≥ 30, ariko umubare wimbuto kuri buri kibanza wabazwe ugereranije kuva ku bimera 15 biva mubibanza bitatu (n = 6).Isesengura mibare ryakozwe hifashishijwe isesengura ryibice bibiri bitandukanye (ANOVA).Inyenyeri zerekana itandukaniro rinini cyane ugereranije no kwigana (* p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001; ns, ntabwo ari ngombwa).HA - aside humic;MZ3, MiZax3, MiZax5;
Hanyuma, twakoresheje isesengura ryibanze (PCA) kugirango dusuzume ingaruka ziterwa n’ibirayi (T x Y) na strawberry (T x C).Iyi mibare yerekana ko kuvura HA bisa na acetone mu birayi cyangwa amazi muri strawberry (Igicapo 8), byerekana ingaruka nkeya ugereranije no gukura kw'ibimera.Igishimishije, ingaruka rusange za MiZax3 na MiZax5 zerekanye ikwirakwizwa rimwe mubirayi (Igicapo 8A), mugihe ikwirakwizwa ryibi bice byombi muri strawberry byari bitandukanye (Ishusho 8B).Nubwo MiZax3 na MiZax5 byagaragaje cyane cyane ikwirakwizwa ryiza mu mikurire y’umusaruro n’umusaruro, isesengura rya PCA ryerekanye ko ibikorwa byo kugenzura imikurire bishobora no guterwa n’ubwoko bw’ibimera.
Isesengura ryibanze ryibanze (PCA) ry (A) ibirayi (T x Y) na (B) strawberry (T x C).Gutanga amanota kubitsinda byombi.Umurongo uhuza buri kintu kiganisha hagati ya cluster.
Muri make, dushingiye ku bushakashatsi bwacu butanu bwigenga bwibihingwa ku bihingwa bibiri bifite agaciro kanini kandi bihuye na raporo zacu zabanjirije iyi kuva 2020 kugeza 202226,27, MiZax3 na MiZax5 byizeza abashinzwe kugenzura ibihingwa bishobora kuzamura imikurire n’umusaruro., harimo ibinyampeke, ibiti bikozwe mu biti (imikindo y'amatariki) n'imbuto z'imbuto z'imbuto 26,27.Nubwo uburyo bwa molekuline burenze ibikorwa byibinyabuzima bikomeje kutoroha, bifite amahirwe menshi yo gukoresha umurima.Icyiza muri byose, ugereranije na acide humic, MiZax ikoreshwa muburyo buto cyane (micromolar cyangwa miligramme) kandi ingaruka nziza ziragaragara.Rero, turagereranya igipimo cya MiZax3 kuri porogaramu (kuva hasi kugeza hejuru cyane): 3, 6 cyangwa 12 g / ha, hamwe na dosiye ya MiZx5: 4, 7 cyangwa 13 g / ha, bigatuma PGRs igira akamaro mukuzamura umusaruro wibihingwa .Birashoboka rwose.
Igihe cyo kohereza: Nyakanga-29-2024