Ni irihe tandukaniro riri hagati ya zeatin, Trans-zeatin na zeatin riboside? Ni ubuhe buryo basaba?

Ibikorwa by'ingenzi

1. Guteza imbere kugabana selile, cyane cyane igabana rya cytoplazme;

2. Guteza imbere itandukaniro. Mu muco wa tissue, ikorana na auxin kugirango igenzure itandukaniro no gushinga imizi nuduti;

3.

4. Gutinda amababi ya senescence, kugabanya umuvuduko wo kwangirika kwa chlorophyll na proteyine;

5. Gabanya imbuto zidasinziriye, gusimbuza urumuri kugirango uhuze urumuri rwimbuto nkitabi;

6. Shira parthenocarpy mu mbuto zimwe;

7.

8. Shishikarizwa gushinga ibirayi.

Irimo gusa imiterere ya trans kandi ifite ingaruka nkizozeatin, ariko hamwe nigikorwa gikomeye.

Ingaruka zayo zirasa cyane na anti-zeatin. Ntabwo ifite imikorere yavuzwe haruguru ya zeatin gusa, ahubwo ifite n'ingaruka zo gukora imvugo ya gene nibikorwa bya metabolike.

Uburyo bwo gukoresha

1. Guteza imbere kumera kwa callus (bigomba gukoreshwa hamwe na auxin), kwibanda 1mg / L.

2. Guteza imbere imbuto, 1001 mg / L zeatin + 5001 mg / L GA3 + 201 mg / L NAA, gutera imbuto ku minsi 10, 25, na 40 nyuma yo kumera.

3. Ku mboga zifite amababi, shyira kuri mg / L 201 kugirango utinde umuhondo wamababi.

Byongeye kandi, kuvura imbuto zimwe zibihingwa birashobora guteza imbere kumera; kuvura murwego rwingemwe birashobora guteza imbere gukura.

1. Guteza imbere kumera kwinyama za callus (bigomba gukoreshwa hamwe na auxin), kuri concentration ya 1 ppm;

2. Guteza imbere imbuto, 100 ppm ya cytokinin + 500 ppm ya GA3 + 20 ppm ya NAA, utere imbuto iminsi 10, 25, na 40 nyuma yindabyo;

3. Gutinda umuhondo wamababi yimboga, gutera 20 ppm;

1. Mu muco wibihingwa, ibisanzwe bya anti-cytokinin nucleoside ni mg / mL cyangwa irenga.

2. Mu kugenzura imikurire y’ibimera, kwibumbira hamwe kwa anti-cytokinin nucleoside ni 1 ppm kugeza 100 ppm, kandi kwibumbira hamwe biterwa nuburyo bwihariye nubwoko bwibimera. Kurugero, mugihe uteza imbere kumera kwinyama za callus, kwibumbira hamwe kwa anti-cytokinin nucleoside ni 1 ppm, kandi bigomba gukoreshwa hamwe na auxin.

3. Kuramo ifu ya anti-cytokinin nucleoside neza hamwe na 2-5 mL ya 1 M NaOH (cyangwa 1 M acide acetike cyangwa 1 M KOH), hanyuma ushyiremo amazi yatoboye kabiri cyangwa amazi ya ultrapure kugirango utegure igisubizo kibitse cya mg / mL cyangwa yibanze cyane. Kangura mugihe wongeyeho amazi kugirango wivange neza. Igisubizo cyo kubika kigomba guhindurwa no gukonjeshwa kubikwa kugirango wirinde gukonjesha. Koresha igisubizo cyo kubika hamwe n'umuco uciriritse usabwa kwibanda, hanyuma utegure igisubizo cyakazi aho kiri hanyuma ukoreshe ako kanya.

Mu gusoza, zeatin, acide abcisic na acide acide nucleotide buriwese afite ibiyiranga mubijyanye nimiterere, ibikorwa nibikorwa bikoreshwa.Mu gusoza, zeatin, acide abcisic na acide acide nucleotide buriwese afite ibimuranga muburyo bw'imiterere, ibikorwa nibikorwa byakazi. Nyamara, byose bikora nkubugenzuzi bwikura ryibimera kandi bigira uruhare runini mugutezimbere imikurire niterambere.